Bacillus anthracis secretory protein protective antigen (PA) is primary candidate for subunit vaccine against anthrax. Attempts to obtain large quantity of PA from Escherichia coli expression system often result in the formation of insoluble inclusion bodies. Therefore, it is always better to produce recombinant proteins in a soluble form. In the present study, we have obtained biologically act...

Bioremediation refers to the use of organisms to remove environmental pollutants. Besides identifying different new plasmids capable of degrading environmental pollutants, many attempts have been taken by the genetic engineers to enhance the bioremediation potential of different microorganisms. Escherichia coli was engineered to convert chlorinated solvents such as trichloro ethylene and the hi...

The cloned gene coding for Bacillus licheniformis penicillinase (penP) was introduced into Escherichia coli in a heat-inducible lambda Qam vector. After induction, significant amounts of penicillinase were synthesized in the new host. The cellular location of the penicillinase was found to be almost exclusively the outer membrane fraction of E. coli, and virtually no soluble penicillinase was f...

Specific carbohydrate recognition in biology is a dynamic process. Thus, supramolecular multivalent scaffolds with dynamic features have been applied to mimic this process. Herein, we prepared DNA-dendron supramolecular fibers and synthesized carbohydrate-oligonucleotide conjugates (C18mannose). Via DNA hybridization, the C18-mannose could be guided onto the fiber platform and form multiple man...

A procedure is described for the purification of the Escherichia coli outer membrane (lipopolysaccharide or L membrane) with flagella still attached. The resulting lipopolysaccharide membrane was in the form of vesicles that had a trilaminar structure in thin section and contained about 55% lipopolysaccharide and 45% protein. T2 or T4 phage preadsorbed to E. coli were found attached to the puri...

The RNA ligase and polynucleotide kinase of bacteriophage T4 are nonessential enzymes in most laboratory Escherichia coli strains. However, T4 mutants which do not induce the enzymes are severely restricted in E. coli CTr5X, a strain derived from a clinical E. coli isolate. We have mapped the restricting locus in E. coli CTr5X and have transduced it into other E. coli strains. The restrictive l...