Establishment of an efficient and reproducible regeneration protocol is one of the basic prerequisites for genetic transformation of any crop plant. In vitro culture of lentil has proven to be difficult. In spite of a number of reports on the regeneration of this plant, very few satisfying and reproducible protocol has yet been reported. This study carried out for investigation of diff...

Establishment of efficient protocol for the induction of somatic embryogenesis and plant regeneration from embryonic axes explants was achieved in Glycine max (L.) Merr. Highest frequency (92.9%) of embryogenic callus formation was obtained from embryonic axes explants of cv. CO-1 on MS media containing 2,4-Dichlorophenoxy acetic acid (2,4-D) and 6-benzyl adenine (BA). Fast growing, yellowish n...

Indoleacetic acid and abscisic acid are present in cereal seeds. It is believed that they play roles in seed maturation and germination. Modification of environmental conditions can inhibit or modify germination and seedling elongation. Our aim is to analyse the role played by IAA and ABA during the anoxia seedlings growth in lack of oxygen comparing rice, Oryza sativa, the only crop plant able...

various plant organ segments including segments from roots (root tip, and root collar), stems, leaves, and also seeds of several hyoscyamus species (i.e. h. pusillus, h. arachnoideus, annual and biennial h. niger from two distinct geographical regions) were cultured on ms media with various growth regulator combinations, to assess the ability of flower production. statistical assessments showed...

Seed dormancy and resistance to decay are fundamental survival strategies, which allow a population of seeds to germinate over long periods of time. Seeds have physical, chemical, and biological defense mechanisms that protect their food reserves from decay-inducing organisms and herbivores. Here, we hypothesize that seeds also possess enzyme-based biochemical defenses, based on induction of th...

Lisianthus (Eustoma grandiflorum L.) is a very popular, ornamental plant. Due to low germination of its seeds, usual techniques for its propagation are not efficient. The present study was aimed to establish callus from explants of different plant parts of Eustoma grandiflorum L. Raf Shinners in B5, LS and MS media with modifications.  The results showed that among different plant parts, leaf e...

Over three consecutive years in central Mississippi, sweetgum (Liquidambar styraci/lua L.) and sycamore (Platanus occidentalis L.) fruits had nearly reached fullsize by late June. Sweetgum seeds were physiologically mature by mid-August, but dry weight increased until late September. As sweetgum seeds matured, the crude fat level rose to 27 percent of seed dry weight. During maturation, concent...

The present study aimed at establishing a complete plant regeneration protocol for Didymopanax morototoni (matchwood), a native Brazilian forest species. Four types of explants (root, shoot, node, and cotyledonary leaves) were obtained from in vitro germinated seeds. In the first step, woody plant medium (WPM) with casein hydrolysate (250 mgL-1 ) and 2,4-D (1.0 and 5.0 mgL-1) were used combined...