the human papillomavirus l1 major capsid protein (hpv l1), the basis of the current vaccines, self-assembles into virus-like particles (vlps). herein, we describe the expression and purification of recombinant hpv16 l1 in e. coli system. the l1 protein was generated in a fused form using an inducible expression system. the recombinant gst-l1 fusion protein migrated as a 82 kda protein in sds-pa...

this paper reviews recent strategies used for increasing specific yield and productivity in high cell density cultures. high cell density cultures offer an efficient means for the economical production of recombinant proteins. however, there are still some challenges associated with high cell density cultivation (hcdc) techniques. a variety of strategies in several aspects including host design...

conclusions in this experimental study, for the first time in iran the expression and purification of this recombinant protein was done successfully. this recombinant protein could be used as a vaccine candidate and diagnostic purpose in subsequent investigations. results the highest expression of the cytochrome p450 cyp141 protein was obtained by the addition of 1 mm of isopropyl β-d-1-thiogal...

Recombinant gene expression is among the most important techniques used both in molecular and medical research and in industrial settings. Today, two recombinant expression systems are particularly well represented in the literature reporting on recombinant expression of specific genes. According to searches in the PubMed citation database, during the last 15 years 80% of all recombinant genes ...

BACKGROUND Decades of work requiring heterologous expression of eukaryotic proteins have shown that no expression system can be considered as the panacea and the appropriate expression strategy is often protein-dependent. In a large number of cases, yeasts have proven to be reliable organisms for heterologous protein expression by combining eukaryotic cellular organization with the ease of use ...

Abstract Leukodystrophy with vanishing white matter (VWM), also called Childhood Ataxia Central Nervous System Hypomyelination, is caused by mutations in the subunits of eukaryotic translation initiation factor, EIF2B1, EIF2B2, EIF2B3, EIF2B4 or EIF2B5. However, little known regarding underlying pathogenetic mechanisms, and there no curative treatment for VWM. In this study, we established firs...

BACKGROUND The dust mite Lepidoglyphus destructor is a major cause of allergic diseases among farmers. We have previously cloned and sequenced two isoforms of the major allergen Lep d 2 (formerly designated Lep d 1) and found significant homology to group 2 allergens of the house dust mite species Dermatophagoides. We now report on the production and characterization of recombinant Lep d 2. O...

The baculovirus expression vector system (BEVS) is widely established as a highly useful and effective eukaryotic expression system. Thousands of soluble and membrane proteins that, in general, are correctly folded, modified, sorted and assembled to produce highly authentic recombinant proteins have been cloned and expressed. This historical chronology and perspective will focus on the original...