Ten mg hydrocortisone (HC) was administered intravenously to a healthy volunteer after a dexamethasone suppression test and HC concentrations were determined from 1-270 min in plasma, plasma water and on erythrocytes. HC was extracted from erythrocyte concentrates with high efficiency by HC-poor plasma or by human or bovine albumin solutions. Determination of HC in the plasma of the volunteer m...

Genetic defects of anion exchanger 1 (AE1) may lead to spherocytic erythrocyte morphology, severe hemolytic anemia, and/or cation leak. In normal erythrocytes, osmotic shock, Cl(-) removal, and energy depletion activate Ca(2+)-permeable cation channels with Ca(2+)-induced suicidal erythrocyte death, i.e., surface exposure of phosphatidylserine, cell shrinkage, and membrane blebbing, all feature...

Glutathione is a tripeptide natural product characterized by non-canonical peptide bond with an amide moiety linking the nitrogen of cysteine to γ-carboxyl glutamate, and found ubiquitously in nature, animals, plants microorganisms. One most abundant biological matrices represented erythrocytes, being glutathione only sulfur-containing mechanism for red blood cell oxidative protection. Several ...

The influence of histamine and quercetin, as well their combined effect on the content in whole blood, superoxide anion radical, sialic acids, sulfhydryl groups, activity catalase, glutathione peroxidase, transferase rat erythrocytes was studied. It established that quercetin at a concentration 0.1 mM causes an increase blood rats, while all other studied concentrations cause significant decrea...

Background: Previous investigations have revealed, cimetidine, a histamine H2-receptor antagonist, show radioprotective effects against gamma- and neutron-induced micronuclei in bone marrow erythrocytes. In this study, the anticlastogenic effects of famotidine and ranitidine, which act similar to cimetidine as histamine H2-receptor antagonists, was investigated. Materials and Methods : Balb/c m...

Background: Although numerous natural or synthetic drugs have been tested for their radioprotective capacity, yet no suitable drug has been introduced for routine clinical use. In this study the radioprotective effect of "a new herbal immunomodulator" commercially known as IMOD, specifically made to decrease the side effects of HIV virus, was investigated on mouse bone marrow cells. M...

Acrylamide (ACR) is a dietary contaminant derived from a wide range of foods through the Maillard-reaction during the cooking process. The present study focused on the hematotoxic effects of ACR and the protective efficacy of Extra Virgin olive oil (EVOO) in alleviating hematotoxicity and oxidative stress in erythrocytes of adult rats. Rats were divided into four groups of six each: group 1, se...

Previously we demonstrated that sickle erythrocytes sedimenting at high densities after gradient centrifugation contain higher levels of surface immunoglobulin bound in vivo in comparison to low-density erythrocytes from the same patient. The present study examines the possibility that binding of autologous IgG to sickle erythrocytes may be associated with the sickling phenomenon. In the presen...

BACKGROUND Erythrocytes are transfused to treat or prevent imminent inadequate tissue oxygenation. 2,3-diphosphoglycerate concentration decreases and oxygen affinity of hemoglobin increases (P50 decreases) with blood storage, leading some to propose that erythrocytes stored for 14 or more days do not release sufficient oxygen to make their transfusion efficacious. The authors tested the hypothe...