Mixed Lineage Kinase 3 (MLK3), also called as MAP3K11 is a tightly regulated MAP3K member but its cellular function is still not fully understood. Earlier we reported post-translational regulation of MLK3 by estrogen (E2) that inhibited the kinase activity and favored survival of ER+ breast cancer cells. Here we report that MLK3 is also transcriptionally downregulated by E2 in ER+ breast cancer...

We used ChIP-Seq to map ERalpha-binding sites and to profile changes in RNA polymerase II (RNAPII) occupancy in MCF-7 cells in response to estradiol (E2), tamoxifen or fulvestrant. We identify 10 205 high confidence ERalpha-binding sites in response to E2 of which 68% contain an estrogen response element (ERE) and only 7% contain a FOXA1 motif. Remarkably, 596 genes change significantly in RNAP...

Two N otre Dame s tu d en ts w ere robbed a t gun point as they m ade their way back to campus early Saturday m orn­ ing, according to Charles Hur­ ley, assistant director of Notre Dame Security. Two male students reported th ey w ere w a lk in g back to cam pus on Eddy S treet, ju s t so u th of th e five p o in ts intersection, when they w ere approached by two men, who dem an d ed th e ir m ...

We present low-dispersion visible spectra of two regions of the 30 Doradus nebula. When corrected for atomic continuum emission and divided by the spectrum of the exciting and illuminating stars, one of the two spectra clearly shows Extended Red Emission (ERE) superimposed on the scattering component. This ERE band peaks around 7270Å and is 1140Å width. HAC grains are found to explain the obser...

Multiple ribonucleases are widely found in living organisms, but the function and regulation of individual ribonucleases are still not clear. In the present study, we found that one oocytic ribonuclease, RC-RNase, is developmentally expressed in the liver and stored in the oocyte of the bullfrog, while another ribonuclease, RC-RNase L1, is constitutively expressed and retained in the liver at a...

Estrogens exert their regulatory potential on gene expression through different nuclear and non-nuclear mechanisms. A direct nuclear approach is the interaction of estrogen with specific target sequences of DNA, estrogen response elements (ERE) or units. EREs can be grouped into perfect and imperfect palindromic sequences with the imperfect sequences differing from the consensus sequence in one...

Because concern over endocrine disrupting reactions caused by chemicals to humans and animals is growing, a rapid and reliable screening assay for endocrine disrupting chemicals is required. We have developed an in vitro screening assay based on a hormone receptor mechanism using a surface plasmon resonance (SPR) sensor. The interaction between an estrogen receptor alpha (ER) and an estrogen re...

1 ABSTRACT In this paper we propose a new line search algorithm that ensures global convergence of the Polak-Ribi ere conjugate gradient method for the unconstrained minimization of nonconvex diierentiable functions. In particular, we show that every limit point produced by the Polak-Ribi ere iteration is a stationary point of the objective function. Moreover, we prove that, asymptotically, the...

Received: 27. 02. 2006. Accepted: 08. 06. 2006. The aim of this study �as to recognize differences in midterm clinical outcome after femoral neck fracture and hip endoprosthesis implantation. A total of 145 patients �ere examined, 32 patients �ith unipolar, 70 �ith bipolar and 43 patients �ith total hip endoprosthesis. The mean values of Harris Hip Score, after 3.8 ± 1.9 years, �ere: 72.1 ± 17....