In order to address the challenges associated with free lipase in organic solvents, including aggregation, poor stability, and low catalytic efficiency, this study developed two types of poly(o-phenylenediamine) microspheres (solid hollow) as supports for immobilizing lipase. The immobilization process utilized an adsorption method, hollow being identified optimal support a 2:5 enzyme-to-suppor...

Agarose-vinyl sulfone (VS) beads have proven to be a good support immobilize several enzymes. However, some enzymes are hardly immobilized on it. This is the case of penicillin G acylase (PGA) from Escherichia coli, which very slowly this (less than 10% in 24 h). enzyme also not significantly adsorbed aminated MANAE-agarose beads, an anionic exchanger. In study, were modified with divinyl (DVS)...

Three mutations on the penicillin acylase surface (increasing the number of Lys in a defined area) were performed. They did not alter the enzyme's stability and kinetic properties; however, after immobilization on glyoxyl-agarose, the mutant enzyme showed improved stability under all tested conditions (e.g., pH 2.5 at 4 degrees C, pH 5 at 60 degrees C, pH 7 at 55 degrees C, or 60% dimethylforma...

Papain a proteolytic enzyme is one of the enzymes which have various applications in regard to food and chemical industry. Papain isolated by cutting or making incision on unripe fruit papaya. more active green fruit. Papaya contains papain will be helpful treating causes trauma, allergies sports injuries also has superior digestion action when compare pepsin pan...

Enzyme stability is critical in biotechnology, pharmaceutical and cosmetic industries. Investigations on this subject have drawn attention because of its practical application. Bromelain is a thiol-endopeptidase, obtained from pineapple (Ananas comosus), known for its clinical and therapeutic applications, particularly to selective burn debridement and improvement of antibiotic action and anti-...

Pressured by antibiotic use, resistance enzymes have been evolving new activities. Does such evolution have a cost? To investigate this question at the molecular level, clinically isolated mutants of the beta-lactamase TEM-1 were studied. When purified, mutant enzymes had increased activity against cephalosporin antibiotics but lost both thermodynamic stability and kinetic activity against thei...

Preorganization of enzyme active sites for substrate recognition typically comes at a cost to the stability of the folded form of the protein; consequently, enzymes can be dramatically stabilized by substitutions that attenuate the size and preorganization "strain" of the active site. How this stability-activity tradeoff constrains enzyme evolution has remained less certain, and it is unclear w...