AIM: This study aimed to determine the content of matrix metalloproteinase-9 (MMP-9) in aqueous humor (AH) eyes children with uveitis and its role during disease.
 MATERIAL AND METHODS: Twenty (20 uveitis) aged from 3 16 yr (11.253.43 on average) three congenital cataract control group were examined. The concentration MMP-9 was determined by enzyme-linked immunosorbent assay (ELISA) using ...

The analysis of historical materials is an essential component cultural heritage conservation. Protein was one the most important and pervasive organic substances in ancient human societies. Through qualitative quantitative examination protein-based materials, it possible to clarify their source functions heritages, investigate manufacturing technology heritage, identify deterioration mechanism...

AIM Determine the optimal cut-off and the use of Fluorescence Polarization Assay (FPA) to improve the detection of brucellosis in individual goats in Argentina. METHODS Sera from 96 goats from a flock with abortion due to B. melitensis biovar 1 were used to assess the efficacy of the FPA to detect brucellosis in goats. FPA results were compared with those of the Buffered Antigen Plate Aggluti...

An enzyme-linked immunosorbent assay (ELISA) for progesterone measurement in serum or plasma samples using penicillinase as label enzyme is reported. A C3 and C11 derivatives of progesterone were prepared and conjugated to bovin serum albumin (BSA). Polyclonal antibody against these two immunogens were prepared in New Zealand white rabbits. Purified Ig fractions of antibodies were immobilized o...

An enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of Bacteroides with the EDTA-released outer-membrane complex from 29 characterized strains of Bacteroides species. With only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the het...

Hantaan virus is the causative agent of severe hemorrhagic fever with renal syndrome. Clinical surveillance for Hantaan virus infection is unreliable, and laboratory verification is essential. The detection of virus-specific immunoglobulin M (IgM) and IgG in serum is most commonly used for the diagnosis of hantavirus infection. Testing of oral fluid samples instead of serum offers many advantag...

A modified phage-enzyme link immunosorbent assay (phage-ELISA) and a phage dot-blot assay specific for hepatitis B surface antigen (HBsAg) and its antibody were developed by using phage display technology. The phageELISA and phage dot-blot assays enabled to detect HBsAg and anti-HBsAg in human sera, and compatible to commercial detection kit. The fusion phages were immobilized onto microtiter p...

there are increasing evidences that show many xenobiotic chemicals (called as endocrine disruptorchemicals edcs) through interfering with endocrine system, have the capability to induce developmentaland reproductive abnormalities in humans and animals. the yolk protein precursor vitellogenin (vtg) hasproved to be a simple and sensitive biomarker for assessing exposure of fish to edcs especially...

Pythium insidiosum causes a life-threatening infection termed pythiosis in humans and other animals. The organism has been identified tropical subtropical environments worldwide. Since 1985, human increasingly reported from Thailand. Seroprevalence studies estimated that 32,000 Thai people had exposed to the pathogen. In 2018, first animal case Thailand was diagnosed horse. Here, we investigate...