Allopurinol, the xanthine oxidase inhibitor, is the only drug available for the treatment of gout. We examined the xanthine oxidase inhibitory activity of some commercially available flavonoids such asepigallocatechin, acacatechin, myricetin, naringenin, daidzein and glycitein by virtual screening and in-vitro studies. The interacting residues within the complex model and their contact types we...

Abstract
 BACKGROUND OF THE STUDY: Β-carotene, flavonoids and phenolic compounds found in methanol extracts of Moringa leaves papaya fruit have high antioxidant activity so that they can be used for antiaging. The concentration solvent is a factor will affect the levels active ingredients extract. This study aims to determine effect derived from extraction on inhibitory elastase hyaluronid...

Alcoholic liver disease (ALD) is a global that manifests in variety of forms, including alcoholic hepatitis, inflammation, fatty liver, and cirrhosis. One the early stages ALD (AFL) disease, which primarily characterized by accumulation lipids inflammation hepatocytes. Guanylate binding protein 5 (GBP5) has been investigated for its involvement progression. The regulatory effects GBP5 on progre...

background & aim: singhnad guggulu is a traditional ayurvedic formula for detoxifying and rejuvenating the joints. the present study was to investigate the α-amylase inhibition and antioxidant activities of aqueous extract of singhnad guggul (aesg). experimental: α-amylase inhibition assay by dinitro-salicylic acid method and antioxidant activity by ferric chelating and ferrous reducing ass...

Alpha-glucosidase inhibitory activity of fifteen Indian medicinal plants has been evaluated by in vitro enzyme assay. Methanol extracts of Cyperus rotundus (tubers), Plumbago zeylanica (root), Symplocos racemosa (bark), and Terminalia arjuna (bark) had displayed 100% inhibition with the IC50

A total of 374 sera, found by enzyme-linked immunosorbent assay (Rubazyme; Abbott Laboratories, North Chicago, Ill.) to have borderline rubella antibody levels, were tested by hemagglutination inhibition. All sera had Rubazyme indexes in the range of 0.500 to 1.499. Rubazyme sensitivity was 59.0%, and specificity was 80.8%. The predictive value of Rubazyme-positive result was 91.4%, and that fo...

An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Sendai virus, a paramyxovirus, is described. The assay was found to be about 20-fold more sensitive than the hemagglutination inhibition assay. Differentiation between virus specific IgG and IgM is possible. The test appears to be especially useful in the study of early events in antibody formation in vivo as well a...

BACKGROUND In evaluating a photochemical treatment process for inactivating parvovirus B19, there lacked simple culture methods to measure infectivity. The recently developed enzyme-linked immunospot (ELISpot) infectivity assay uses late-stage erythropoietic progenitor cells and is labor intensive and time consuming. We evaluated a novel, efficient polymerase chain reaction (PCR) inhibition ass...

Neurological disorders are characterized by progressive loss of neurons leading to disability. Neurotransmitters such as nor-adrenaline, dopamine, and serotonin partially regulated the enzyme monoamine oxidase (MAO). Treatments for conditions like Alzheimer’s, Parkinson’s, anxiety, depression involve use MAOIs. To target MAO inhibition, various scaffolds prepared evaluated, including modified c...