Immobilization of Lipase produced from Rhizomucor miehei on HDPE fine powder was investigated. As compared to an aqueous system, immobilization in a non-aquous organic medium such as n-hexane was not successful and caused enzyme denaturation. Prewetting the support with ethanol increased the immobilized protein and enzyme activity as much as 31% and 34%, respectively. The maximum immobilized a...

despite the availability of various alkaline phosphatase (alp) isoenzymes, the calf enzyme is being used in current enzyme assays as the detector enzyme. the glycosylation pattern of this enzyme makes it a suitable ligand for binding to wheat germ agglutinin lectin (wga). as a result of this property, the enzyme can not be used as a conjugate with this lectin, and the calf enzyme conjugates can...

background while hepatitis e virus (hev) mostly causes self-limited disease in general population, it is more severe in pregnant women. objectives this study aimed to investigate the seroprevalence of anti-hev igg among a population of pregnant women in west azerbaijan of . patients and methods one hundred thirty six pregnant women referred to urban health centers of urmia for pursuing pregnanc...

materials and methods a total of 228 fecal samples were collected from residents of rural areas of hamadan, west of iran. each fecal sample was divided into two parts, one kept frozen at -20˚c for ag-capture elisa and the other in 10% formalin for the ap staining method. cryptosporidium ag-detection elisa procedure was performed according to the manual of the manufacturer. the preserved samples...

background: central renin angiotensin system has an important role on the cerebral microcirculation and metabolism. our previous work showed that inhibition of angiotensin converting enzyme (ace) activity prior to induction of ischemia protected the brain from severe ischemia/reperfusion (i/r) injuries. this study evaluated the impacts of post-ischemic inhibition of ace, enalapril, on brain inf...

the use of proteins and peptides as human therapeutics has been increased in recent years. lipase is a relatively homogeneous proteinaceous enzyme indicated in maldigestion. the purpose of the present study was to evaluate the effect of humidity along with compactional pressure on the enzymatic activity of wheat germ lipase. samples of lipase powder were kept at different relative humidity (rh)...

zero tillage practices have a direct effect on soil microbial communities modifying soil productivity and sustainability. the crop sequences used can change the aforementioned properties, too. in this study, we evaluated the effect of crop sequences under zero tillage management on soil biological and chemical properties including vertical distribution of soil organic carbon, soil basal respira...

background and objectives: sodium dodecyl sulfate (sds) is one of the main surfactant components in detergents and cosmetics, used in high amounts as a detergent in products such as shampoos, car wash soap and toothpaste. therefore, its bioremediation by suitable microorganisms is important. alkylsulfatase is an enzyme that hydrolyses sulfate -ester bonds to give inorganic sulfate and alcohol. ...