* Supported in part by a grant from the Atomic Energy Commission, Contract No. AT (30-1) 1017. t Public Health Research Fellow of the National Cancer Institute. 1 Yanofsky, C., J. Biol. Chem., 223, 171 (1956). 2 Yanofsky, C., and M. Rachmeler, Biochim. et Biophys. Acta, 28, 640 (1958). 3 DeMoss, J. A., M. Imai, and D. M. Bonner, Bact. Proc., 112 (1958). 4Yanofsky, C., Biochem. et Biophys. Acta,...

A compositional generic process control model is presented which has been applied to control enzymatic biochemical processes. The model has been designed at a conceptual and formal level using the compositional development method DESIRE, and includes processes for analysis, planning and simulation. It integrates qualitative and quantitative techniques. Its application to enzymatic chemical proc...

Copoazú is a fruit originated from Colombian Amazon belonging to Theobroma genus, which includes species such as cacao raw material for chocolate. Both presented similar processing stages, with different process parameters due the physical qualities of each fruit. Enzymatic, mechanical, and combined methods pulping copoazú seeds their effect on biochemical dynamics fermentation were evaluated. ...

In this article, we report on the use of miniaturized and automated enzymatic assays as an alternative technology for fast sugar and acid quantification in apples and tomatoes. Enzymatic assays for d-glucose, d-fructose, sucrose, D-sorbitol/xylitol, L-malic acid, citric acid, succinic acid, and L-glutamic acid were miniaturized from the standard 3 mL assays in cuvettes into assays of 200 microL...

A novel, inexpensive and versatile ionic-liquid-based catch and release mass spectrometry tag (I-Tag) that facilitates substrate purification, fast, robust and sensitive enzymatic reaction monitoring and quantitative kinetic analysis has been developed. The applicability of the system has been demonstrated in an enzymatic assay with β-1,4-galactosyltransferase.

Objective(s):Hyperglycemia mediated oxidative stress plays a key role in the pathogenesis of diabetic complications like nephropathy. In the present study, we evaluated the effect of ethanolic extract of Ensete superbum seeds (ESSE) on renal dysfunction and oxidative stress in streptozotocin-induced diabetic rats. Materials and Methods:Glucose, HbA1c, total protein, albumin, renal function mar...

in the present study a new economic and rapid bacterial micro-assay for simultaneous detection and quantitative measurement of serum galactose was developed. analysis of the standard curve showed a linearity range for galactose from 2 mg/dl to 180 mg/dl with a regression equation of y = 0.013x ? 0.083; r² = 0.962. the advantage of the method is its ability to measure serum galactose quantitativ...

this study utilized molecular methods to identify 22 isolates from traditional dairy products of azerbaijan. pcr was carried out using the universal primers of 16s rdna and a fragment of ~1500bp was amplified. amplified ribosomal dna restriction analysis was performed using bsuri, mspi, hindiii, taqi and bsp143i. ardra results were compared to the restriction profile in enzymatic restriction an...

A method is described for the initial steps of sequence analysis of RNase T1-and pancreatic RN-ase-resistant oligonucleotides of RNA containing cytidylate residues labeled in vitro with 125I. In many cases an oligonucleotide sequence can be deduced from a consideration of (i) its relative position in the two-dimensional fingerprint (with DEAE thin layer homochromatographic second dimension), (i...