BACKGROUND AND OBJECTIVE Direct demonstration of Entamoeba histolytica by conventional microscopy and in vitro culture in pus obtained from amebic liver abscess (ALA) is often unsuccessful. We evaluated polymerase chain reaction (PCR) for detection of E. histolytica DNA in such pus. METHODS Species-specific primers were used for the amplification of E. histolytica DNA from liver pus obtained ...

We have developed a reliable method for PCR-based genotyping of Entamoeba histolytica based on variation in the numbers of short tandem repeats that are linked to tRNA genes in this species. Species-specific primer pairs were designed that differentiate E. histolytica from E. dispar as well as that reveal intraspecies PCR product length polymorphisms. The primers were tested with samples from d...

introduction: differential diagnosis of potential pathogenic entamoeba histological from non-pathogenic entamoeba dispar by a molecular method. material and methods: one pair of primers designed based on sequence of genomic dna coding the 30kd surface antigen of e.histolytica/e.dispar. a 374 base pair pcr product was amplified by using the primers. electrophoretic patterns of pcr product digest...

Data on the epidemiology of Entamoeba infections in Eastern part Kenya is scanty. Diagnostic tests use have limited capacity to differentiate common infecting species. The performance Polymerase Chain Reaction (PCR) between pathogenic histolytica and two nonpathogenic species E. dispar moshkovskii largely undetermined. Therefore, this study sought determine prevalence associated factors for eva...

monkeys are important experimental models for investigating human diseases. the aim of this study was to survey the alimentary tract parasites among imported vervet monkeys (cercopithecus aethiops) to iran. fecal samples were collected from 40 vervets imported from tanzania to razi vaccine and serum research institute of iran. fecal samples were assessed by direct smear and telman sedimentation...

abstract intestinal parasitic infections are among the most important infectious diseases that are associated with individual and public hygiene. every year millions of people are infected with some sort of intestinal parasites worldwide. in iran there are different types of intestinal parasites that cause economic, social, and health losses. the aim of the cross-sectional study was to investig...

A growth-promoting factor (GPF) that promotes the growth of Entamoeba dispar under axenic culture conditions was found in fractions of mitochondria (Mt), hydrogenosomes (Hg) and chloroplasts (Cp) obtained from cells of six different protozoan, mammalian and plant species. We were able to extract the GPF from the Cp-rich leaf cells of a plant (spiderwort: Commelina communis L.) in an acetone-sol...