نتایج جستجو برای: embryonic stem es cell

تعداد نتایج: 1909995  

2014
Tokunori Ikeda Shinya Hirata Koutaro Takamatsu Miwa Haruta Hirotake Tsukamoto Takaaki Ito Makoto Uchino Yukio Ando Seiho Nagafuchi Yasuharu Nishimura Satoru Senju

We herein demonstrate the immune-regulatory effect of embryonic stem cell-derived dendritic cells (ES-DCs) using two models of autoimmune disease, namely non-obese diabetic (NOD) mice and experimental autoimmune encephalomyelitis (EAE). Treatment of pre-diabetic NOD mice with ES-DCs exerted almost complete suppression of diabetes development during the observation period for more than 40 weeks....

Journal: :Journal of visualized experiments : JoVE 2009
Brad Hamilton Qiang Feng Mike Ye G Grant Welstead

Using a defined set of transcription factors and cell culture conditions, Yamanaka and colleagues demonstrated that retrovirus-mediated delivery and expression of Oct4, Sox2, c-Myc, and Klf4 is capable of inducing pluripotency in mouse fibroblasts.(1) Subsequent reports have demonstrated the utility of the doxycycline (DOX) inducible lentiviral delivery system for the generation of both primary...

2009
Herbert Schulz Raivo Kolde Priit Adler Irène Aksoy Konstantinos Anastassiadis Michael Bader Nathalie Billon Hélène Boeuf Pierre-Yves Bourillot Frank Buchholz Christian Dani Michael Xavier Doss Lesley Forrester Murielle Gitton Domingos Henrique Jürgen Hescheler Heinz Himmelbauer Norbert Hübner Efthimia Karantzali Androniki Kretsovali Sandra Lubitz Laurent Pradier Meena Rai Jüri Reimand Alexandra Rolletschek Agapios Sachinidis Pierre Savatier Francis Stewart Mike P. Storm Marina Trouillas Jaak Vilo Melanie J. Welham Johannes Winkler Anna M. Wobus Antonis K. Hatzopoulos

Embryonic stem (ES) cells have high self-renewal capacity and the potential to differentiate into a large variety of cell types. To investigate gene networks operating in pluripotent ES cells and their derivatives, the "Functional Genomics in Embryonic Stem Cells" consortium (FunGenES) has analyzed the transcriptome of mouse ES cells in eleven diverse settings representing sixty-seven experimen...

2012
Young-Eun Kim Jeong-A Park Yang-Wha Ha Sang-Kyu Park Hee Sun Kim Sun Kyung Oh Younghee Lee

Human embryonic stem (ES) cells are a potential source of cells for developmental studies and for a variety of applications in transplantation therapies and drug discovery. However, human ES cells are difficult to culture and maintain at a large scale, which is one of the most serious obstacles in human ES cell research. Culture of human ES cells on MEF cells after disassociation with accutase ...

Journal: :Differentiation; research in biological diversity 2011
Florence Wianny Pierre-Yves Bourillot Colette Dehay

Non-human primate (NHP) embryonic stem (ES) cells show unlimited proliferative capacities and a great potential to generate multiple cell lineages. These properties make them an ideal resource both for investigating early developmental processes and for assessing their therapeutic potential in numerous models of degenerative diseases. They share the same markers and the same properties with hum...

2008
Tzung K Hsiai Joseph C. Wu

Pluripotent embryonic stem can (ES) cells can differentiate into all cell lineages. During the process of embryonic development, ES cells are exposed to fluid flow or blood flow generated by the contracting heart. Absence of fluid flow results in the formation of abnormal cardiac chambers and valve formation. Thus, hemodynamic forces and ES cell differentiation to vascular progenitor cells (VPC...

Journal: :The International journal of developmental biology 2006
Erik Willems Ileana Mateizel Caroline Kemp Greet Cauffman Karen Sermon Luc Leyns

Embryonic Stem (ES) cells have the potential to form every cell of the body and thus are of great promise for tissue transplantation. One of the rising techniques that allows studying the differentiation state of ES cells is quantitative RT-PCR (qRT-PCR). When relative quantification by qRT-PCR is applied, accurate normalization is necessary, since differentiated embryonic stem cells and develo...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2004
Xiaoxia Qi Teng-Guo Li Jing Hao Jie Hu Jing Wang Holly Simmons Shigeto Miura Yuji Mishina Guang-Quan Zhao

The fate of pluripotent stem cells is tightly controlled during early embryonic development. Both the derivation and the maintenance of embryonic stem cells (ES cells) in vitro depend on feeder cell-derived growth factors that are largely unidentified. To dissect the mechanisms governing pluripotency, we conducted a screen to identify factors that are produced by mouse embryonic fibroblast STO ...

Journal: :Acta oto-laryngologica 2008
Mark Praetorius Ignacio Vicario Thomas Schimmang

CONCLUSION Cell transplantation into the utriculus provides an efficient and non-invasive route to introduce embryonic stem (ES) cells into the vestibular and cochlear portions of the inner ear. OBJECTIVE The transfer of stem cells into the inner ear for therapeutic purposes is an important approach to cure damage to the cochlea and vestibulum. A key issue is to provide an entry point for cel...

Journal: :Stem cells 2009
Doyeob Kim Sanjeevkumar R Patel Hong Xiao Gregory R Dressler

Pax transactivation domain-interacting protein (PTIP) is a ubiquitously expressed, nuclear protein that is part of a histone H3K4 methyltransferase complex and is essential for embryonic development. Methylation of H3K4 is an epigenetic mark found on many critical developmental regulatory genes in embryonic stem (ES) cells and, together with H3K27 methylation, constitutes a bivalent epigenetic ...

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