Many plant cells can be reprogrammed into a pluripotent state that allows ectopic organ development. Inducing totipotent states to stimulate somatic embryo (SE) development is, however, challenging due to insufficient understanding of molecular barriers that prevent somatic cell dedifferentiation. Here we show that Polycomb repressive complex 2 (PRC2)-activity imposes a barrier to hormone-media...

the aim of this study was to analyze the incidence of stage of embryonic development, embryo quality and the time of transfer on pregnancy rate in embryo transfer program timed. we were worked a total of 23holsteincowsasembryo donors, super-ovulated with gonadotropin of pregnant mare serum (pmsg) (2500 3000ui).69 cows were used as recipients of holstein dairy heifers with an age of20 months and...

A PCR-based genomic scan has been undertaken to estimate the extent and ratio of maternally versus paternally methylated DNA regions in endosperm, embryo, and leaf of Zea mays (maize). Analysis of several inbred lines and their reciprocal crosses identified a large number of conserved, differentially methylated DNA regions (DMRs) that were specific to the endosperm. DMRs were hypomethylated at ...

A 42 year old with primary infertility over 2 years with poor ovarian reserve (AMH 0.02) with an Oligoastheno-terato-zoospermia had 3 attempts of embryo transfer with her own egg. USG showed a heterotopic pregnancy with fundal 5-6 weeks intrauterine gestational sac with a cervical ectopic with small subchorionic collection along with posterior uterine wall. Despite embryo reduction, it still sh...

March 8 is a member of a family of transmembrane E3 ubiquitin ligases that have been studied mostly for their role in the immune system. We find that March 8 is expressed in the zebrafish egg and early embryo, suggesting a role in development. Both knock-down and overexpression of March 8 leads to abnormal development. The phenotype of zebrafish embryos and Xenopus animal explants overexpressin...

Assisted reproductive technology has made great progress during the last three decades. After the initial enthusiasm, many ethical, legal and social issues related to the application of these procedures began to evolve. Multifetal pregnancy and fetal reduction, embryo cryopreservation, preimplantation genetic diagnosis, risks of birth defects and other adverse outcome associated with assisted r...

In pea, high temperatures during reproductive development lead to severe yield loss. Although the ovule is seed precursor, studies elucidating effect of heat on this plant structure are scarce. We investigated impact in field and growth chamber ovules 4 days after open flower (4DOF) stage. Objectives were identify associations between ovaries performance, evaluate set abortion heat-treated plan...

Embryo culture were used to produce inter specific walnut hybrid plants between Juglans nigra L. and J. regia L. Developed ovaries were collected from J. rejia female flowers which were pollinated with J. nigra pollen grain by high dusting on a mature tree. In order to isolate embryos, exocarps were aseptically removed. Isolated embryos from ovules at different developmental stages were transfe...

objective: in recent years, antiviral drugs have been used widely in the glob to prevent and treat viral diseases. they injected into eggs to eliminate pathogens and prevent of egg transmission of disease, but the adverse effects of drugs have always been a major concern. there is little information available about the safety of antiviral drugs in the embryonated eggs of birds. the objective of...

Abstract Generally, orchids produce dust-like seeds in which endosperm reduction and embryo undifferentiation represent a derived state shared with species about 11 other plant families. Orchid are proposed to have special kind of morphological or morphophysiological dormancy. We test this proposition, overcoming several design limitations earlier studies, specifically that the vitro germinatio...