Based on experiments with cultured fibroblasts, the apoptosis regulators caspase-9 and Apaf-1 are hypothesized to function as tumor suppressors. To investigate their in vivo role in lymphomagenesis, an IgH enhancer-driven c-myc transgene was crossed onto Apaf-1(-/-) and caspase-9(-/-) mice. Due to perinatal lethality, Emu-myc transgenic Apaf-1(-/-) or caspase-9(-/-) fetal liver cells were used ...

Evidence is presented that the growth capacity of chick embryo tissue for the fowl coryza bodies is conditioned by a diffusible cellular component which is essential for their multiplication. This growth factor is inactivated at pH 6, but withstands a temperature of 100 degrees C. for 60 minutes. An amount sufficient to promote a normal growth of the specific bodies may be present in tissue cul...

Comparison of the binding to DNA of 7-hydroxymethyl-12-methylbenza(a)anthracene and 7, 12-dimethylbenz(a)anthracene (DMBA) catalyzed by mouse embryo cells in culture or by rat liver microsomes indicates that the products formed are different for the two hydrocarbons. Thus, the hydroxy compound is not an intermediate in the binding of DMBA to DNA in these systems. Binding of the hydroxy compound...

Syrian hamster embryo fibroblasts transformed in vitro with benzo(a)pyrene were analyzed for the presence of type C viral components, including extra- and intracellular reverse transcriptase activity, intracellular type C hamster virus-related RNA, and cellular hamster virus group-specific antigen. No evidence could be obtained for the presence of any of these components, although they were eas...

1. Heart and liver tissue samples were obtained from rats in various developmental stages from the 12-day-old embryo to the 120-day-old postnatal animal. 2. The body, heart and liver weights and percentage protein in the liver and heart of the prenatal and postnatal rat were determined. 3. The activities of NADH-, NADPH- and succinate-cytochrome c reductases and cytochrome oxidase were determin...

Methods A 31-year-old primigravid woman presented with a twin pregnancy at 14 weeks of gestation for evaluation of structural abnormalities in one co-twin. The marriage was non consanguineous. There was not history of genetic disorders or structural anomalies noted in the both parents. This was her first pregnancy and it was achieved by in vitro fertilization (ivf) and embryo transfer. One embr...

A new technique for the intracellular localization of minute amounts of heme and hemoproteins is described. The specimen is treated with 1.5 M perchloric acid in the presence of SH groups, followed by ultraviolet light irradiation in a fluorescence microscope. This fixes the proteins in situ and converts the heme to a porphyrin which fluoresces and is readily visualized. With this technique, he...

BACKGROUND: There are several reports that indicate a linkage between exposure to power frequency (50 - 60 Hz) magnetic fields with abnormalities in the early embryonic development of the chicken. The present study was designed to understand whether power frequency electromagnetic fields could act as an environmental insult and invoke any neurochemical or toxicological changes in developing chi...

the society for assisted reproductive technology (sart) method of embryo grading is unique, simple, and widely practiced, and its use has been mandatory for sart membership programs since 2010. developed by sart in 2006, the current embryo grading system categories, “good, fair, and poor,” are limited because they do not describe the best 1-2 embryos in the interest of keeping pace with the shi...

In this study we have investigated by real time polymerase chain reaction (RT-PCR) the expression of cytokines, which are well known angiogenic and anti-angiogenic molecules, during chick embryo development in four organs, namely the chorioallantoic membrane (CAM), heart, liver and optic tectum at four different stages. We have studied the expression of vascular endothelial growth factor-A (VEG...