hydatid cyst is a world wide and zoonotic disease between human and animal. in this study 384 buffalos were used for blood sampling according to cocran formula. in compliance with elisa kit to study the samples, the needed antigen was removed from the fertile hydatid cysts and purified according to oriol and rogan method.then reliability of the conjugate (biodesign company) required density of ...

Concerns were raised about specificity of the VIKIA Rota-Adeno immunochromatographic kit. Only 28-37% of samples positive with the VIKIA kit could be confirmed using two real-time RT-PCR assays and three ELISA kits. On re-analysis of a subset of the positive samples, 86% remained positive with the VIKIA kit, however, 90% remained negative in the other assays. In a highly vaccinated population w...

AIM: This study aimed to determine the content of matrix metalloproteinase-9 (MMP-9) in aqueous humor (AH) eyes children with uveitis and its role during disease.
 MATERIAL AND METHODS: Twenty (20 uveitis) aged from 3 16 yr (11.253.43 on average) three congenital cataract control group were examined. The concentration MMP-9 was determined by enzyme-linked immunosorbent assay (ELISA) using ...

Background: The purpose of this study is to evaluate effects of Teucrium polium (TP) leaf extracts on adenosine monophosphate activated protein kinase (AMPK) level in isolated pancreases. Materials and Methods: In this experimental study, two groups of rats were used as a control group, and diabetic group. In the end of experimental period the animals were sacrificed then pancreas were removed...

Hepatitis E virus (HEV) infects both humans and animals, with an overall human mortality rate generally less than 1%, but as high as 20% among pregnant women. HEV strains fall into 4 major genotypes. Zoonotic genotypes 3 and 4 associate with sporadic human and animal HEV cases in many industrialized countries. To date, collective evidence implicates pigs as the main HEV reservoir, justifying th...

cystic hydatid disease (hydatidosis) is one of the most important zoonosis that is caused by the larval stage of echinococcus granulosus . as its diagnosis by clinical symptoms alone is difficult and confusing, serologic diagnostic techniques are used to confirm the disease. these techniques can also be used for   epidemiologic studies. the present study was performed with a commercial human en...

AIM To detect and identify the causative agent or agents of the following clinical symptoms which were fever, lack of appetite, salivation, vesiculation, erosions of the buccal mucosa, nose, and feet. The signs vary from mild to severe. The mortality rate of the disease is high. The morbidity rate reaches up to 100%. Sheep also show bloody diarrhea and rapid respiration. Sheep flock resident in...

We have developed a kit to diagnose hydatidosis, based on the detection of specific antibodies. This disease, caused by larvae of the cestode Echinococcus granulosus, is a zoonosis commonly found throughout the world. The diagnostic kit discriminates between positive and negative cases in a way that is easily interpreted, i.e., positive cases form a colored band on an inert substrate. The resul...

Background: An accessible and sensitive method for determining antibodies to a new coronavirus infection is often the key timely provision of necessary medical care patients with rheumatic diseases Objectives: Compare methods SARS-CoV-2 using rapid test enzyme-linked immunosorbent assay (ELISA) Methods: Methods an express (Chromatographic IgG / IgM (Xiamen Biotime Biotechnology, China)) by ELIS...

Reportedly, complexes between factor XI and ADAMTS13 are detected with a commercial ADAMTS13/FXI ELISA kit in plasma and are decreased in thrombotic thrombocytopenic purpura (TTP). Using this kit, control and TTP patient plasma contained varying amounts of signal (25-670% of a reference plasma) but no signal was observed for mixtures of recombinant enzymes, suggesting little interaction. ADAMTS...