A methodologic study was performed to compare the polymerase chain reaction (PCR) and Southern blot hybridization, two commonly used testing strategies for the detection of human papillomavirus (HPV) infection. Three laboratories tested masked aliquots of exfoliated cervical cell specimens obtained from 120 women by cervicovaginal lavage. The study population included 32 women with condylomatou...

This report describes the detailed procedures for Western blot analysis using fluorescent antibodies. After electrophoresis and subsequent electroblotting, the fluorescent-labeled antibodies were visible upon ultraviolet illumination of the polyvinylidene fluoride (PVDF) membranes and could then be photographed to give an accurate record of the blots. Fluorescent labeling allows for photographi...

nucleocapsid (n) protein of infectious bronchitis virus (ibv), one of the viral structural proteins, inducesstrong antibody response in natural infection. in this study, a simple, recombinant n protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of ibv antibody.initially, 72 serum samples were tested for the presence of ibv antibody using a c...

ticks are important ectoparasites which are a considerable threat to human beings and to animals all overthe world. enormous economic losses annually occur in livestock production around the world as a result oftheir existance. one of the ways to control ticks and tick-borne diseases is to introduce resistance to theseectoparasites through immunization. for identification of the putative protec...

in this paper, we investigate variation of the wavelength, intensity and polarization of the self-assembled inas/gaas quantum dots emission by microphotoluminescence spectroscopy at the liquid helium temperature. the microcavity wafer sample is grown by molecular beam epitaxy (mbe) and chemically etched into the micropillar structure (with elliptical cross section - long and short axis 2µm×1.5µ...