Whether or not a single gene is transcribed relies on a myriad of stochastic factors which may not be adequately described by the cell’s genome alone. Understanding the connection between the occupancy of a cell’s chromatin and the transcription of its genes would provide insight into the dynamic regulatory dependencies that control its internal transcription state, and so enhanced techniques f...

The expression of eukaryotic genes is regulated by cis-regulatory elements such as promoters and enhancers, which bind sequence-specific DNA-binding proteins. One of the great challenges in the gene regulation field is to characterise these elements. This involves the identification of transcription factor (TF) binding sites within regulatory elements that are occupied in a defined regulatory c...

The effect of Friend disease virus infection on spleen acid nuclease activity was studied in dejtail. RNase activ ity decreased rapidly, with time, during the course of the virus-induced leukemogenesis. Within 3 to 12 days after infection there was an 80 to 90% or greater suppression of RNase activity, as calculated per mg spleen tissue or protein. RNase activity remained depressed throughout t...

An endonuclease named DNase gamma has been purified from the nuclei of apoptotic rat thymocytes [Shiokawa, Ohyama, Yamada and Tanuma (1997) Biochem. J. 326, 675-681]. Here we report the molecular cloning of a cDNA encoding a 35 kDa precursor protein for rat DNase gamma. A 1.6 kb mRNA coding for the DNase gamma precursor is detected at high levels in spleen, lymph nodes, thymus and liver. By usi...

A spectrophotometric assay is described for staphylococcal nuclease, based on the increase in absorbance at 260 mp which accompanies deoxyribonucleic acid and RNA hydrolysis. Initial velocities are proportional to enzyme concentration over a 70-fold range. The enzyme has greater aflinity for DNA than for RNA, and activity is greater with heat-denatured DNA than with native DNA. No inhibitory pr...

The objective of this research was to determine the presence virulence genes (asa1, gelE, cylA, ace, esp, hyl and efaA), vancomycin resistance (vanA, vanB, vanC2/C3) some antibiotics Enterococcus spp. isolates previously identified from different stages ripened white cheese production. In addition, gelatinase, β-hemolytic DNase activity, biofilm formations were examined phenotypically. study, e...