In the course of various biological processes, specific DNA-binding proteins must efficiently find a particular target sequence/protein or damaged site on DNA. perform this task based diffusion. Nevertheless, investigations over recent decades have found that diffusion dynamics are generally complicated and, further, protein specific. review, we collect experimental and theoretical studies quan...

Background: Deoxyribozymes (Dzs) can play a role as gene expression inhibitors at mRNA level. Among Dzs, the 10-23 deoxyribozyme has significant potentials for treatment of diseases. Designed Dz includes a catalytic core made of 15 deoxyribonucleotides and two binding arms consisted of 6-12 nucleotides for site specific binding to target RNA and hydrolysis. The enzyme has characteristic feature...

Canola (Brassica napus L.) is an important oilseed crop. A serious problem in cultivation of this crop andyield loss, are due to fungal disease stem rot caused by Sclerotinia sclerotiorum. The pathogenesis-related(PR) proteins have the potential for enhancing resistance against fungal pathogen. Thaumatin-like proteins(TLPs) have been shown to have antifungal activity on variou...

Background: Spine gourd (Momordica dioica Roxb. Willd) is one of the important cucurbitaceous crops grown across the world for vegetable and medicinal purposes. Diseases caused by the DNA viruses are becoming the limiting factors for the production of spine gourd reducing its potential yield. For the commercial cultivation of the spine gourd, propagation material used by most o...

Over the last decade, our knowledge of phylogenetic and taxonomic diversity coralline algae has greatly progressed thanks to use DNA sequence data. Despite this, in some geographic re...

Many recent studies have shown that glycosylation patterns of Agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. To achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. To isolate this promoter, PCR with specific primers was perform...

Background: Recent researches have demonstrated that human T-cell immunoglobulin mucin 1 (TIM-1) glycoprotein plays important roles in regulation of autoimmune and allergic diseases, as well as in tumor immunity and response to viral infections. Therefore,targeting TIM-1 could be a potential therapeutic approach against such diseases. Objectives: In this study, we aimed to express TIM-1 protein...