نتایج جستجو برای: dna primers

تعداد نتایج: 520069  

1999
Melanie S. Arendse Ian A. Dubery David K. Berger

A polygalacturonase-inhibiting protein (pgip) gene from Malus domestica cv Granny Smith apple fruit was cloned by degenerate oligo-primed polymerase chain reaction (PCR) and Inverse PCR. An alignment of the pear and bean PGIP sequences was used to design degenerate PCR primers in highly conserved regions. Degenerate PCR allowed the amplification of a 351bp internal fragment of the pgip gene, te...

Journal: :Applied and environmental microbiology 1997
K Groppe T Boller

A PCR assay which allows detection and quantification of Epichloë endophytes in tissues of the grass Bromus erectus is described. PCR with specific primers flanking a microsatellite-containing locus (MS primers) amplified fragments 300 to 400 bp in length from as little as 1.0 pg of fungal genomic DNA in 100 ng of DNA from infected plant material. When annealing temperatures were optimized, all...

Journal: :BioTechniques 2005
Yair Adereth Kristen J Champion Tien Hsu Vincent Dammai

Here we describe a simple and cost-effective PCR-based method to introduce deletions, point mutations, frame-shift, or truncations with very high efficiency. The scope and additional applications of the method are discussed. Site-directed mutagenesis involves the introduction of mutations at the DNA level to alter the primary amino acid sequence of proteins (1,2). To approach the desired level ...

2017
Jennifer Lu Kelin Ru Ida Candiloro Alexander Dobrovic Darren Korbie Matt Trau

Multiplex bisulfite-PCR sequencing is a convenient and scalable method for the quantitative determination of the methylation state of target DNA regions. A challenge of this application is the presence of CpGs in the same region where primers are being placed. A common solution to the presence of CpGs within a primer-binding region is to substitute a base degeneracy at the cytosine position. Ho...

Journal: :Journal of virology 1974
A J Faras J E Dahlberg R C Sawyer F Harada J M Taylor W E Levinson J M Bishop H M Goodman

The 70S RNA of Rous sarcoma virus contains 4S RNAs which serve as primers for the initiation of DNA synthesis in vitro by the RNA-directed DNA polymerase of the virus. We purified these primers in three different ways-by isolation of the covalent complex between primer and nascent DNA, by differential melting of the 70S RNA, and by two-dimensional electrophoresis in polyacrylamide gels. The 4S ...

2009
Rudolph Spangler Noel L. Goddard David S. Thaler

BACKGROUND PCR in principle can detect a single target molecule in a reaction mixture. Contaminating bacterial DNA in reagents creates a practical limit on the use of PCR to detect dilute bacterial DNA in environmental or public health samples. The most pernicious source of contamination is microbial DNA in DNA polymerase preparations. Importantly, all commercial Taq polymerase preparations ine...

Journal: :Nucleic acids research 1992
N Akopyanz N O Bukanov T U Westblom S Kresovich D E Berg

The RAPD (or AP-PCR) DNA fingerprinting method was used to distinguish among clinical isolates of Helicobacter pylori, a bacterium whose long term carriage is associated with gastritis, peptic ulcers and gastric carcinomas. This method uses arbitrarily chosen oligonucleotides to prime DNA synthesis from genomic sites to which they are fortuitously matched, or almost matched. Most 10-nt primers ...

Journal: :Metabarcoding and Metagenomics 2021

The unprecedented ongoing biodiversity decline necessitates scalable means of monitoring in order to fully understand the underlying causes. DNA metabarcoding has potential provide a powerful tool for accurate and rapid monitoring. Unfortunately, many cases, lack universal standards undermines widespread application metabarcoding. One most important considerations plants, aside from selecting p...

2008
Alexandre V. Lebedev Natasha Paul Joyclyn Yee Victor A. Timoshchuk Jonathan Shum Kei Miyagi Jack Kellum Richard I. Hogrefe Gerald Zon

The polymerase chain reaction (PCR) is widely used for applications which require a high level of specificity and reliability, such as genetic testing, clinical diagnostics, blood screening, forensics and biodefense. Great improvements to PCR performance have been achieved by the use of Hot Start activation strategies that aim to prevent DNA polymerase extension until more stringent, higher tem...

Journal: :Biological Invasions 2022

Abstract The early detection of invasive species is essential to cease the spread before it can cause irreversible damage environment. analysis environmental DNA (eDNA) has emerged as a non-harmful method detect presence visual and promising approach monitor species. Few studies have investigated use eDNA for arthropods, their exoskeleton expected limit release into We tested published primers ...

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