Plasposons are modified mini-Tn5 transposons for random mutagenesis of Gram-negative bacteria. Their unique design allows for the rescue cloning and sequencing of DNA that flanks insertion sites in plasposon mutants. However, this process can be laborious and time-consuming, as it involves genomic DNA isolation, restriction endonuclease treatment, subsequent religation, transformation of religa...

thaliana plants. C. R. Acad. Sci. (Paris) 316:1194-1199. 2.Devic, M., S. Albert, M. Delseny, and T.J. Roscoe. 1997. Efficient PCR walking on plant genomic DNA. Plant Physiol. Biochem. 35:331-339. 3.Doyle, J.J. and J. L Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13-15. 4.Frey, M., C. Stettner, and A. Gierl. 1998. General method for gene isolation in tagging approaches: Ampli...

Plastid DNA has been isolated from many dif­ ferent sources and characterized in terms of bouyant density, base composition, renaturation kinetics, and sedimentation velocity (for references, c f . 1' 2). For many years, however, it was not possible to isolate plastid DNA in a form sufficiently intact to determine its molecular weight by length measure­ ments in electron micrographs. Only recen...

Procedures are described for the detection and isolation of plasmids of various sizes (2.6 to 350 megadaltons) that are harbored in species of Agrobacterium, Rhizobium, Escherichia, Salmonella, Erwinia, Pseudomonas, and Xanthomonas. The method utilized the molecular characteristics of covalently closed circular deoxyribonucleic acid (DNA) that is released from cells under conditions that denatu...

Semen cryopreservation is an import toll for reproductive management of fish species, which increasingly it is applied in the commercial production of fish. Cryopreservation need of the cryoprotectors agents (CPAs) that may be a complication factor in DNA extraction that can affect the quality of isolated DNA. Genomic DNA constitutes the total genetic information of an organism and it is used f...

DNA isolation is a crucial step of conducting genetic studies in any organism. However, this process is quite difficult when studying fungi because of the need to damage the fungal cell walls of specific structures. In this study, we developed a method for the rapid and efficient isolation of fungal DNA based on simultaneous mechanical and enzymatic cell wall degradation. There are several typi...

As an inanimate virus, herpes simplex virus type 1 (HSV-1) necessarily encodes all of its functions in its DNA. Isolation of pure viral DNA allows multiple downstream applications, including the creation of recombinant HSV strains, cloning of selected regions, and sequencing of viral DNA. The term nucleocapsid refers to the combination of the viral genome with the enclosing capsid; these viral ...

We describe simple, inexpensive, and reliable methods for isolating DNA from avian blood, semen, or feather pulp. The procedures are readily applicable to high-throughput 96-well plate isolation for genotype analysis of chicken DNA based on restriction endonuclease digestion or PCR. Isolation cost is primarily the cost of a deep-well assay block and a few pipet tips; current price is less than ...

The isolation of p53 immunostain positive cells from histological sections for molecular genetic studies is a difficult task, especially if there are few positive cells. To eliminate contaminating DNA from p53 negative cells, which can obscure the results of molecular assays, a variation on the technique of immunohistoselective sequencing was developed. This is a highly selective approach, wher...

Recently, several works have taken advantage of a database isolation notion suitable for replicated approaches, called Generalized Snapshot Isolation, that provides greater performance since read-only transactions are never blocked nor cause update transactions to block or abort. However, this concept has not been formally described for replicated environments where a logical copy of the system...