Mitochondrial DNA from wild-type Saccharomyces cerevisiae and from an "extreme" petite mutant were analyzed by hybridization of several tRNAs on DNA fragments of different buoyant density, obtained by sonication and fractionation on a CsCl gradient. The hybridization patterns show that the genes for tRNAser, tRNAphe, tRNAhis, tRNAval, tRNAileu are present on wild-type mitochondrial DNA, while o...

In the last period, Steganography is commonly used as an alternative to encryption achieve secret communication between parties. Many methods have emerged steganography, including use of spatial domain, spread spectrum, transform and etc. On other hand, attackers also developed in revealing hidden data trying retrieve it. To increase security hiding process, some researchers found hybrid that c...

Spinach ( Spinacia oleracea Linnaeus, 1753) is an ideal material for studying molecular mechanisms of early-stage sex chromosome evolution in dioecious plants. Degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) technique facilitates the retrotransposon-relevant studies by enriching specific repetitive DNA sequences from a micro-dissected single chromosome. We conducted genomi...

A highly sensitive and selective method for amplified electrochemical detection for hairpin-stem-loop structured target sequences was developed based on the temperature regulation of DNA hybrids on a sandwich-type electrochemical DNA sensor. Multistep hybridization was applied to promote the hybridization efficiency of each section of sandwich structure. The results showed that both multistep a...

Deoxyribonucleic acid-ribonucleic acid (DNA-RNA) hybridization studies have been performed with R-plasmid DNA (R538-1drd) and in vivo-synthesized RNA. R-plasmid DNA was isolated from Escherichia coli K-12, and the complementary strands were separated in cesium chloride-polyuridylic acid-polyguanylic acid gradients. DNA-RNA hybridization was performed with the separated DNA strands and RNA purif...

Deoxyribonucleic Acid (DNA) has certain unique properties such as selfassembly and self-complementary in hybridization, which are important in many DNAbased technologies. DNA computing, for example, uses these properties to realize a computation in vitro, which consists of several chemical reactions. Other DNA-based technologies such as DNA-based nanotechnology and polymerase chain reaction als...

The hybridization of complementary nucleic acid strands is the most basic of all reactions involving nucleic acids, but has a major limitation: the specificity of hybridization reactions depends critically on the lengths of the complementary pairs of strands and can drop (in the presence of other competing strands whose sequences are close to that of a given target strand) to very low values if...