In this study the programmed cell death (apoptosis) of human neutrophilic granulocytes was investigated in the presence and absence of fetal calf serum (FCS), cycloheximide and actinomycin-D. The results show that when FCS is omitted from cultures, apart from a decrease in viability, the percentage of apoptotic cells and DNA fragmentation increases. Apoptosis is accelerated in serum withdra...

Rapid and extensive target cell DNA fragmentation is a unique characteristic of CTL-mediated killing. We studied the role of the granule pore-forming protein (PFP/perforin/cytolysin) of CTL in mediating lysis and DNA fragmentation of target cells. Perforin was isolated from murine CTL by sequential application of perforin-enriched granule fractions to four chromatographic columns: DEAE-Sepharos...

the pathogenesis of equine grass sickness (egs) has not fully understood. a better understanding of the exact pathogenesis of diseases can help to make an accurate diagnosis. previous studies reported some pathological damage of neuronal cells in egs patients. in this study, primarily cytotoxicity of serum from three clinically egs-diagnosed horses on pc12 tet-off (pto) cells was assessed. subs...

objective(s): regarding the presence of many active biological constituents in avicennia marina, the present investigation was carried out to study cytotoxic activity of crude methanol leave extract and column chromatographic fractions of a. marina against mda-mb 231 cell line (human breast cancer cell) and hek (human embryonic kidney cell) line .   materials and methods: the anticancer activit...

background: the present study aimed to survey antileishmanial activity of methanolic holothuria leucospilota extract against leishmania major promastigotes in vitro. methods: promastigotes were cultured in rpmi 1640 and after reaching the stationary phase, the study was conducted with different concentrations of the extract. afterwards, mtt colorimetric assay for the obtaining of 50% inhibitory...

The present study revealed the damaging effects of copper and hydrogen peroxide on DNA isolated from human blood in in vitro. Ultra violet spectral studies showed that copper and H2O2 alone (at 20 mM) caused destabilization of DNA structure. Notwithstanding, the effect was more prominent in combination of copper and H2O2. Further, agarose gel electrophoretic studies revealed that neither copper...

Background Sperm preparation before assisted reproductive techniques is very important for obtaining successful fertilization and pregnancy. During ICSI technique, sperms are selected based on viability and morphology. But this selection did not guarantee integrity of sperm DNA. Therefore, novel sperm selection procedures are introduced for selection of sperm with low DNA damage. One of these p...

Mycophenolic acid (MPA) is the active metabolite of mycophenolate mofetil. This study designed to investigate the mechanism of cytotoxicity of MPA on the genetically engineered PC12 Tet Off (PTO) neuronal cells with p53 gene. Alamar Blue (AB) reduction showed concentration-dependent cytotoxicity of MPA on PTO cells with IC50 value of 32.32 ± 4.61 mM. The reactive oxygen species (ROS) generation...