A review is presented of recently developed methods for quantifying electron microscopical thin sections on which colloidal gold-labelled markers are used to identify and localize interesting molecules. These efficient methods rely on sound principles of random sampling, event counting, and statistical evaluation. Distributions of immunogold particles across cellular compartments can be compare...

Continuous labelling of callus with H-thymidine results in intermittent peaks of H-DNA per chloroplast, showing synchrony of division. The increase in H-DNA could be due to several replication rounds, and the drop to successive plastid divisions without intervening DNA synthesis. The level of DNA-binding proteins in the chloroplast parallels the peaks of plastidal DNA synthesis; such pro...

Various 13CO� labelling approaches exist to trace carbon (C) dynamics in plant-soil systems. However, it is not clear if the different approaches yield the same results. Moreover, there is no consistent way of data analysis to date. In this study we compare with the same experimental setup the two main techniques: pulse and continuous labelling. We evaluate how these techniques perform to estim...

Differential protein expression analysis based on modification of selected amino acids with labelling reagents has become the major method of choice for quantitative proteomics. One such methodology, two-dimensional difference gel electrophoresis (2-D DIGE), uses a matched set of fluorescent N-hydroxysuccinimidyl (NHS) ester cyanine dyes to label lysine residues in different samples which can b...

BACKGROUND Low knowledge of and discrimination regarding mental disorders (MDs) may underpin lower access to mental health care by ethnic minority groups. AIMS In Chinese-Australians, in relation to schizophrenia and depression, to assess (a) labels attached to MDs, (b) conceptual distinctiveness of MDs, (c) labelling accuracy against an Australian representative sample, (d) how syndrome vari...

We examine decompositions of complete graphs with an even number of vertices, K2n, into n isomorphic spanning trees. While methods of such decompositions into symmetric trees have been known, we develop here a more general method based on a new type of vertex labelling, called flexible q-labelling. This labelling is a generalization of labellings introduced by Rosa and Eldergill.

Various 13CO� labelling approaches exist to trace carbon (C) dynamics in plant-soil systems. However, it is not clear if the different approaches yield the same results. Moreover, there is no consistent way of data analysis to date. In this study we compare with the same experimental setup the two main techniques: pulse and continuous labelling. We evaluate how these techniques perform to estim...

There is an increasing interest in semantically annotated 3D models, e.g. of cities. The typical approaches start with the semantic labelling of all the images used for the 3D model. Such labelling tends to be very time consuming though. The inherent redundancy among the overlapping images calls for more efficient solutions. This paper proposes an alternative approach that exploits the geometry...