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Angiotensin-converting enzyme can be measured by the rate of release of 3H-labelled hippurate from p-[3H]benzoylglycylglycylglycine. The product is separable from the substrate by extraction of acidified reaction mixtures with ethyl acetate. Assay results for human serum angiotensin-converting enzyme can be obtained within 1.5 h of receipt of serum samples. Within the limits tested, the assay a...
Acute reactions to the haemodialysis membrane have been reported as early as 1975 [1]. Because their severity is usually mild, their frequency is probably widely underestimated. However, these reactions may occasionally be life-threatening [2]. The aetiological mechanisms of these reactions are multiple, the best characterized being allergy to ethylene oxide and combined exposure to the polyacr...
Many of us recognize that some individuals seem "gifted" in sporting ability. We may also have noted the association of such elite performance with past parental success, recognizing intuitively the role of inherited traits. With the expansion of molecular biology and associated technologies, we now find ourselves better able to explore these genetic influences. This article examines the role o...
Title: New perspectives in the renin-angiotensin-aldosterone system (RAAS) II: albumin suppresses angiotensin converting enzyme (ACE) activity in human Authors: Miklós Fagyas, Katalin Úri; Ivetta M. Siket; Gábor Á. Fülöp; Viktória Csató; Andrea Daragó, Judit Boczán, Emese Bányai, István Elek Szentkirályi, Tamás Miklós Maros, Tamás Szerafin, István Édes, Zoltán Papp, Attila Tóth Affiliations: Di...
Expression of CD4, the principle receptor for HIV-1, is not sufficient for viral entry into most non-human and rare human cell lines. Construction of HIV-1 susceptible heterokaryons and a hybrid cone by fusion of HeLa cells and the HIV-1 resistant human cell line U373-CD4 were previously reported by us. These results suggested that U373-CD4 lack a cofactor(s) which is essential for HIV-1 entry ...
It is now clear that angiotensin-converting enzyme (ACE) inhibitors have an important role in the management of patients after myocardial infarction. Several clinical trials have shown statistically significant reductions in mortality (SAVE, AIRE, GISSI-3, ISIS4).1-4 Some may argue that the weight of evidence favours wide, if not universal, use of ACE inhibitors among post-infarct patients. Doe...
BACKGROUND Salivary endothelin (ET) concentrations have been shown to correlate with disease severity in patients with chronic heart failure (CHF). We undertook the present study to evaluate the stability of salivary ET under different handling conditions to assess its suitability as a biochemical marker in screening, diagnosis, and management of CHF. METHODS Saliva samples were collected fro...
Genotyping increases the yield of angiotensin-converting enzyme in sarcoidosis--a systematic review.
INTRODUCTION The diagnosis of sarcoidosis is challenging and involves radiological, clinical and paraclinical evaluation, the latter including the measurement of serum angiotensin-converting enzyme activity (s-ACE), which is elevated in about 60% of sarcoidosis patients. The normal inter-individual biological variation of s-ACE is large. Approximately 50% of the variation is due to a genomic in...
Ibuprofen is metabolized to chemically reactive acyl glucuronide and S-acyl-CoA metabolites that are proposed to transacylate glutathione (GSH) forming ibuprofen-S-acyl-GSH (I-SG) in vivo. Herein, we report the detection of novel metabolites of ibuprofen, namely ibuprofen-N-acyl-cysteinylglycine (I-N-CG), ibuprofen-N-acyl-cysteine (I-N-C), and the mercapturic acid conjugate, ibuprofen-S-acyl-N-...
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