In order to obtain information on the mechanism of ether bond formation in the synthesis of 0-alkyl lipids, a study was carried out to determine if there is a loss of hydrogen from carbon 3 of dihydroxyacetone phosphate (DHAP) (nonphosphorus-linked carbon) in the course of its incorporation into glyceryl ethers. Accordingly, a mixture of [1,3-3H2] DHAP and [I ,3-WJDHAP was prepared. In a micros...

It is generally accepted that the mechanism of enzyme activity includes a combination of enzyme and substrate. This concept forms the basis for the conventional kinetic analyses of enzymatic reactions (1). Direct evidence for the existence of enzyme-substrate combinations is as yet meager. The binding of pyridine nucleotide coenzymes as substrates to various dehydrogenases has been shown to res...