a method to quick detect concentration of lecithin in eggs, namely microwave digestion spectrophotometry, was established in this research. the homogenate of eggs was treated with absolute ethanol to eliminate phosphate protein in eggs which could possibly affect concentration of lecithin examined. a sample then received a new way of pre-treatment, called microwave digestion, before uv-vis spec...

We design moving horizon state estimators for a general model of bioprocesses. The underlying optimization is nonconvex due to the microbial growth kinetics, which are modeled as nonlinear functions. relax constraints so that becomes second-order cone program, can be solved efficiently at large scales. Unfortunately, solutions relaxation inexact and thus lead inaccurate estimates. To recover fe...

Our objective was to characterize changes in cell wall composition and digestibility of sugarcane bagasse, pith from bagasse, and wheat straw after treatment with alkaline hydrogen peroxide (AHP). The AHP treatment solution contained 1% H2O2 (wt/vol) maintained at pH 11.5 with NaOH. The H2O2 in solution amounted to 25% of the quantity of substrate treated. After treatment, residues were washed ...

Methanosarcina species with a high maximum specific growth rate (mumax) and high half-saturation coefficient (KS) and Methanosaeta species with a low mumax and low KS are the only known aceticlastic methanogens. Because of Methanosaeta's low KS, the low acetate concentrations in conventional, mesophilic anaerobic digestion yield Methanosaeta dominance. However, Methanosarcina absorbs increases ...

The fermentation of the neutral detergent-soluble (NDS) fraction of two legumes (clover, alfalfa) and two grasses (timothy, guinea grass) was measured using a curve subtraction technique with in vitro gas production data from the whole forage and the isolated neutral detergent-extracted fiber. The NDF disappearance and VFA production also were measured. There were no significant differences bet...

Combined anaerobic–aerobic sludge digestion has been demonstrated as a valid technological solution to improve the sludge stabilization process, in terms of solid reduction and sludge dewaterability properties. In this study, we investigated the fate of all nitrogen species (ammonia, nitrites, nitrates) in the sequential digestion process when the post-aerobic step is operated with intermittent...

Solid wastes from the meat industry are produced in large amounts resulting in a negative impact on the environment if not properly treated. Due to their high content of proteins and fats, these residues are excellent substrates for anaerobic digestion which holds high potential for methane yield. However, possible toxic compounds may be formed during its biodegradation with a consequent failur...

Mass spectrometry (MS) coupled to affinity purification is a powerful approach for identifying protein-protein interactions and for mapping post-translational modifications. Prior to MS analysis, affinity-purified proteins are typically separated by gel electrophoresis, visualized with a protein stain, excised, and subjected to in-gel digestion. An inherent limitation of this series of steps is...

The use of grafted trypsin magnetic beads in a microchip for performing protein digestion is described. The PDMS device uses strong magnets to create a magnetic field parallel to the flow with a strong gradient pointing through the center of the chip channel. This allows for the formation of a low-hydrodynamic resistance plug of magnetic trypsin beads that serves as a matrix for protein digesti...