Ca2+-free calmodulin was obtained by exhaustively dialyzing 2 mg protein (c = 4 mg/mL) against 1000 volumes of 2 mM EGTA, 50 mM Tris HCl and 150 mM NaCl (pH = 7.4) at 4 °C in a 0.5 mL Pierce Slide-A-LyzerTM Dialysis Cassette (3.5 K MWCO) for 2 days. As the carboxylic groups of EGTA exhibit strong IR bands in the region 1570-1630 cm-1 that overlap with the amide I' band of proteins (1600-1700 cm...

Residual renal function (RRF) in patients with end-stage renal disease (ESRD) receiving renal replacement therapy is defined as the ability of native kidneys to eliminate water and uremic toxins. Preserved RRF improves survival and quality of life in adult ESRD patients treated with peritoneal dialysis. In children, RRF was shown not only to help preserve adequacy of renal replacement therapy b...

Background: Dialysis adequacy is one of the most important factors in determining the survival rate and mortality of dialysis patients. Objectives: The aim of this study was to determine the dialysis adequacy of patients undergoing chronic hemodialysis at dialysis centers located in the province of Zanjan, located in northwest Iran. Methods: This descriptive cross-sectional study was performe...

Nguyen, Minhtri K., and Ira Kurtz. A new formula for predicting alterations in plasma sodium concentration in peritoneal dialysis. Am J Physiol Renal Physiol 288: F1113–F1117, 2005; doi:10.1152/ajprenal.00387.2004.—Alterations in the plasma water sodium concentration ([Na ]pw) result from changes in the total exchangeable sodium (Nae), total exchangeable potassium (Ke), and total body water (TB...

Dialysis unphysiology was first discussed by Carl Kjellstrand in 1975 for the possible negative effects of the unphysiology of intermittent dialysis treatment. Current hemodialysis practices are still unphysiologic because they cannot keep blood chemistries within normal limits, both before and after dialysis. In addition, the discontinuous nature of hemodialysis causes saw-tooth volume fluctua...

BACKGROUND In clinical practice, dietary sodium assessment requires reliable and rapid screening tools. We wished to evaluate the usefulness of food frequency questionnaires (FFQ) in estimating dietary sodium intakes in haemodialysis patients. METHODS We used the Derby Salt Questionnaire (DSQ), and Scored Sodium Questionnaire (SSQ) to estimate sodium intake. Body composition was determined by...

Drug release from liposomal formulations is governed by a complex interplay of kinetic (i.e., drug permeability) and thermodynamic factors (i.e., drug partitioning to the bilayer surface). Release studies under sink conditions that attempt to mimic physiological conditions are insufficient to decipher these separate contributions. The present study explores release studies performed under nonsi...

A high throughput, low volume microfluidic device has been designed to decouple the physical processes of protein crystal nucleation and growth. This device, called the Phase Chip, is constructed out of poly(dimethylsiloxane) (PDMS) elastomer. One of the Phase Chip's innovations is to exploit surface tension forces to guide each drop to a storage chamber. We demonstrate that nanoliter water-in-...

We present a radiometric assay for the determination of urinary angiotensin-converting enzyme activity, using benzoyl-[1-14C]glycyl-L-histidyl-L-leucine as the substrate. An optimal pH of 8.3, an optimal chloride concentration of 0.375 mol/l and complete inhibition by EDTA-Na2, captopril and enalaprilat confirm the specificity of the assay. Comparison of dialysis and ultrafiltration for concent...

Large amounts (200 mg per liter of culture supernatant fluid) of highly purified staphylococcal soluble delta hemolysin were obtained by adsorption to and selective elution from hydroxyapatite followed by exhaustive dialysis against water, concentration by polyvinylpyrrolidone or polyethylene glycol 20,000 dialysis, and a final water dialysis. No carbohydrate, phosphorus, or inactive 280-nm abs...