Prostate cancer cells adhere to a tumor basement membrane, while secretory epithelial cells reside in a suprabasal cell compartment. Since tumor cells are derived from suprabasal epithelial cells, they experience de-novo substratum adhesion in the context of oncogenesis. We therefore analyzed whether cell-matrix adhesion could affect the protein expression and activity of the AR. In this study,...

Development of serum-free suspension cell culture processes is very important for influenza vaccine production. Previously, we developed a MDCK suspension cell line in a serum-free medium. In the present study, the growth kinetics of suspension MDCK cells and influenza virus production in the serum-free medium were investigated, in comparison with those of adherent MDCK cells in both serum-cont...

Hypericum triquetrifolium Turra. is a valuable medicinal plant owing to source of many bioactive compounds. Hypericin is one of the significant components among these compounds. The aim of this study was to develope an efficient method allowing to improve hypericin production from calli and cell suspension cultures of Hypericum triquetrifolium Turra. Callus formation was obtained from axenic le...

MDCK cells are currently being considered as an alternative to embryonated eggs for influenza virus propagation and hemagglutinin (HA) production intended for vaccine manufacturing. MDCK cells were found suitable for the virus production but their inability to grow in suspension burdens the process of scale up and hence their production capability. Anchorage-dependent MDCK cells were converted ...

Strategies are needed to improve repopulation of decellularized lung scaffolds with stromal and functional epithelial cells. We demonstrate that decellularized mouse lungs recellularized in a dynamic low fluid shear suspension bioreactor, termed the rotating wall vessel (RWV), contained more cells with decreased apoptosis, increased proliferation and enhanced levels of total RNA compared to sta...

We have found that Ehrlich ascites tumour (EAT) cells, deprived of any carbon source, and suspended at a density of 2 X 10(5) cells/cm3, begin to die only after 12 h of starvation, though it is known that under these conditions they lose over 80% of their ATP within 30 min. Moreover, we have found that the viability of the cells incubated in the absence of any substrate for energy metabolism is...

Colloidal processing of ceramics is reviewed with an emphasis on interparticle forces, suspension rheology, consolidation techniques, and drying behavior. Particular attention is given to the scientific concepts that underpin the fabrication of particulate-derived ceramic components. The complex interplay between suspension stability and its structural evolution during colloidal processing is h...

3E. L. Post, J. Symb. Logic, 12, 1-11, 1947. 4In the sense of Post, the word problem for Z is reducible to that for sp (2:). 5 W. Magnus, Math. Annalen, 106, 295-307, 1932. 6 DanaScott, abstract, J. Symb. Logic, 21, 111-112, 1956. 7Marshall Hall, J. Symb. Logic, 14, 115-118, 1949. 8 G. Higman, B. H. Neumann, and H. Neumann, J. London Math. Soc., 24, 247-254, 1949. 9 As defined, UG, Part I, p. 2...

A highly sheared dense aqueous suspension of granular cornstarch particles displays rich nonlinear rheology. We had previously demonstrated the growth and onset interfacial instabilities when shear-thinning suspensions were displaced by a Newtonian fluid, suggested methods to maximise displacement efficiency [Palak, R. Sathayanath, S. K. Kalpathy Bandyopadhyay, Colloids Surf. Physicochem. Eng. ...