Dichlorodiammineplatinum(ll) is an anti-neoplastic agent that is currently undergoing clinical evaluation. We describe an analytical method for monitoring the free drug (or its breakdown products) in plasma. The method is able to distinguish between free and protein-bound drug. Plasma samples are deproteinized by centrifugal ultrafiltration. The platinum in the ultrafiltrate is converted to a c...

PURPOSE of this report is to present a rapid method for the determination of urea in blood and urine based upon the urea-pdimethylaminobenzaldehyde reaction. Ehrlich, Rhode, Brocher, Weltmann and Barrenscheen, and Werner (1-5) reported on numerous substances that form red, orange, violet, and yellow interaction complexes with p-dhnethylaminobenzaldehyde. Activated carbon has the specific proper...

Abstract Objectives The present study was performed to determine if minocycline HCl could influence the behavior of deproteinized bovine bone mineral (DBBM) and bioactive glass (BG) particles when used as filler material for new generation in a guided augmentation model. Materials Methods Two occlusive titanium caps were placed on rat calvaria. One filled with BG particles, second DBBM both pre...

Cation-selective exhaustive injection and sweeping followed by a MEKC separation is evaluated for the sensitive analysis of 5-nitroimidazoles in untreated human serum and urine. Deproteinized serum and urine samples were diluted 76 and 143 times, respectively, in a low-conductivity solvent (5.00 mM orthophosphoric acid containing 5.0% v/v methanol). Samples were electrokinetically injected at 9...

Shrimp chitin and chitosan with improved characteristics were isolated from black tiger shrimp waste pretreated with 0.04 M Salicylic acid for ten hours. The pretreated shrimp shells could be efficiently demineralized and deproteinized at ambient temperature using 0.680 M HCl and 0.620 M NaOH, respectively. The duration of the treatments was 8 hours, the ash and protein residues in the final ch...

Phosphorylation is one of the main post-translational modification (PTM) involved in signaling network in the ascomycete Botrytis cinerea, one of the most relevant phytopathogenic fungus. The data presented in this article provided a differential mass spectrometry-based analysis of the phosphoproteome of B. cinerea under two different phenotypical conditions induced by the use of two different ...

We describe a fully automated method of analysis for plasma epinephrine and norepinephrine by use of a two-column system of "high-performance" liquid chromatography. Catecholamines in deproteinized plasma are purified on the first (preparation) column, then transferred automatically to the second (analytical) column in which epinephrine and norepinephrine are resolved. These compounds are then ...

Metronidazole and its known metabolites in plasma can be rapidly separated by a "high-pressure" liquid-chromatographic method that can also be adapted for rapid determination of tinidazole. Samples deproteinized with trichloroacetic acid (50 g/L final concentration) undergo isocratic separation on a reversed-phase C18 column eluted with an 8/92 (by vol) mixture of acetonitrile/KH2PO4 (5 mmol/L,...

Bovine spermatozoa have been shown to metabolize exogenous substrates both aerobically and anaerobically (Mann, 1964). Fructose (Mann, 1946) and sorbitol (King & Mann, 1958) have been unequivocally identified in seminal plasma, but evidence for the presence of metabolizable substrates in the cervical mucus rests on much less secure foundations. A number of reports that glucose is present in cer...

We demonstrate a liquid-chromatographic method involving post-column derivatization for determining the concentration of thiamin and its phosphate esters in human blood. Blood, erythrocytes, or plasma is deproteinized and centrifuged. Aliquots of the samples are applied to a mu Bondapak C18 column attached to a "high-performance" liquid chromatograph. Addition of potassium ferricyanide/sodium h...