Received April 9, 1963 We have previously reported (Heath and Ghalambor, 1963) the occurrence of 2-keto-3-deoxy-octonate @DO) as a glycosidically-bound constituent of cell wall lipopolysaccharide (LPS) of Rscherichia coli 0111-B& and the enzymatic synthesis of CMP-RDO (Ghalambor and Heath, 1963). This report presents evidence for the chemical synthesis of KDO and its enzymatic cleavage (RDO-ald...