Stimulation with live dengue virus of peripheral blood mononuclear cells from a dengue virus type 4-immune donor generated virus-specific, serotype-cross-reactive, CD8+, class I-restricted cytotoxic T lymphocytes (CTL) capable of lysing dengue virus-infected cells and cells pulsed with dengue virus antigens of all four serotypes. These CTL lysed autologous fibroblasts infected with vaccinia vir...

Chemical and physical variables influencing the plaquing of all dengue serotypes in two simian cell systems were studied. Calf serum in the nutrient overlay may be replaced by mouse ascitic fluid or bovine plasma albumin when employing the rhesus monkey kidney LLC-MK(2) cell system for plaquing all dengue serotypes. Doubling the serum concentration in the overlay had little effect in modifying ...

All four serotypes of dengue have been cotransmitting in the country for many years [2]. However, they do not occur in equal abundance. In a series of 477 dengue tests done in Colombo in 2007, among 55 positive detections, the serotypes detected were DEN-1 in 1 (1.8%); DEN-2 (dengue virus serotype 2) in 25 (40.0%), and DEN3 in 19 (34.5%). DEN-4 (dengue virus serotype 4) was not detected. The se...

While dengue virus is thought to replicate in mononuclear phagocytic cells in vivo, attempts to detect it in peripheral blood mononuclear cells (PBMC) by virus isolation or antigen detection have had variable and generally low rates. In this study, we developed a reverse transcription (RT)-real-time PCR assay to quantify positive- and negative-sense RNA of dengue virus type 2 within the cells. ...

A total of 4,243,049 dengue cases have been reported in Brazil between 1981 and 2006, including 5,817 cases of dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) and a total of 338 fatal cases. Although all Brazilian regions have been affected, the Northeast and Southeast regions have registered the highest number of notifications. DENV-1 and DENV-4 were isolated for the first time in the...

Abstract. Datu AM, Natzir R, Yustisia I, Wahid Soraya GV, Kadir S. 2023. Molecular detection and analysis of dengue virus genetic diversity in North Sulawesi, Indonesia during 2022. Biodiversitas 24: 3407-3413. Dengue is a highly infectious disease caused by the virus, consisting four serotypes (DENV1-DENV4). The existence diverse genotypes an area significantly impacts degree virulence humans,...

BACKGROUND Delhi, a city in north India, has so far witnessed several reported outbreaks of dengue. Dengue in Delhi from being epidemic is slowly changing towards being endemic and hyper-endemic. Circulating type of the virus is also changing over the years. In the absence of an effective vaccine, dengue prevention to a major extent relies on virological surveillance, and development of effecti...

Metabolites isolated from marine inverteimmunodeficiency virus (1, 5, 6, 7), were tested on a new in vitro bioassay using the dengue virus 1. Only brates, callipeltin A 1, crambescidin 2, ptilomycalin A 3, gymnochrome D and isogymnochrome D isolated from celeromycalin 4, gymnochrome B 5, gymnochrome D 6 the living fossil crinoid Gymnocrinus richeri are highly and isogymnochrome D 7 previously s...

The principal aim of this work was to report the reintroduction of dengue virus serotype 3 in the Americas after an absence of 17 years. In addition, it describes the most common symptoms associated with classical dengue and hemorrhagic dengue and presents data on the distribution of the epidemic in the various comprehensive local health care systems of Nicaragua. The study group consisted of 3...

DNA microarrays were used to assess the innate gene signature in human myeloid dendritic cells infected with chimeric dengue 1-4 vaccines, a wild-type dengue 3 virus, or a classically attenuated serotype 3 vaccine shown to be reactogenic in humans. We observed a very reproducible signature for each of the 4 chimeric dengue vaccines, involving stimulation of type I interferon and associated gene...