Transcriptional regulation depends upon the binding of transcription factor (TF) proteins to DNA in a sequence-dependent manner. Although many experimental methods address the interaction between DNA and proteins, they generally do not comprehensively and accurately assess the full binding repertoire (the complete set of sequences that might be bound with at least moderate strength). Here, we d...

A kinetic and equilibrium binding study of the interaction of 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25-(OH)2D3) with its receptor system from chick intestinal mucosa has been carried out. The equilibrium dissociation constant (Kd) for the binding of 1 alpha,25-(OH)2D3 to the crude cytoplasmic receptor varies from 2.3 x 10(-10) M at 4 degrees C to 5.0 x 10(-10) M at 24 degrees C, yielding valu...

Morpho-phonological patterns and semantic density influence the processing of spoken complex words contribute to dissociation between regularly irregularly inflected forms. However, it is unclear whether all listeners rely on morpho-phonological cues same degree. The present paper examines a listener’s cognitive profile, indicated by efficiency, affects strategy employed when listening morpholo...

this study aimsto investigate an aspect of marital interactions, distance. by applying aqualitative method and conducting 43 in-depth semi-structured interviews, including 22women and 21 men, women and men experiences of distance were investigated based on theirown narratives.the results of the study suggested different forms of distance in couples' relationships,which can be classified to...

To examine the existence of negative cooperativity among formyl peptide chemotaxis receptors, steady-state binding of f Met-Leu-[3H]Phe to viable rat neutrophils and their purified plasma membranes was measured and the data were subjected to statistical analysis and to computer curve fitting using the NONLIN computer program. Curvilinear, concave upward Scatchard plots were obtained. NONLIN and...

The aim of this work is to develop a procedure for determining the ion dissociation degree and electron density in supersonic jet gas-discharge source collisionless plasma from results measurements current collected by an insulated probe system with transversely oriented cylindrical electrodes. Based on mathematical model collection asymptotic solution saturation region obtained previously, new...

Humans have the abilities to perceive, produce, and synchronize with a musical beat, yet there are widespread individual differences. To investigate these abilities and to determine if a dissociation between beat perception and production exists, we developed the Harvard Beat Assessment Test (H-BAT), a new battery that assesses beat perception and production abilities. H-BAT consists of four su...