DNA adenine methylase mutants of Salmonella typhimurium contain reduced amounts of FinP, an antisense RNA encoded by the virulence plasmid pSLT. Lowered FinP levels are detected in both Dam- FinO+ and Dam- FinO- backgrounds, suggesting that Dam methylation regulates FinP production rather than FinP half-life. Reduced amounts of F-encoded FinP RNA are likewise found in Dam- mutants of Escherichi...

This paper presents experimental and numerical investigations of the seismic failure reinforced unreinforced monoliths Huangdeng concrete gravity dam. To verify scale factors, we use suitable materials (emulation material fine alloy wire) to simulate dam steel reinforcement (SR) in a scaled experiment model that includes water-retaining monolith overflow We design shaking table tests based on s...

In this study, we demonstrated that the methyltransferase activity associated with Dam was essential for attenuation of Aeromonas hydrophila virulence. We mutated aspartic acid and tyrosine residues to alanine within the conserved DPPY catalytic motif of Dam and transformed the pBAD/damD/A, pBAD/damY/A, and pBAD/damAhSSU (with the native dam gene) recombinant plasmids into the Escherichia coli ...

Electromyogram (EMG) radio telemetry was used in conjunction with physiological biopsy to relate prior physiological condition and subsequent swimming energetics and behaviours to passage success of 13 wild adult sockeye salmon Oncorhynchus nerka at a vertical-slot fishway on the Seton River, British Columbia. At the time of capture, plasma lactate, glucose and cortisol levels indicated that fi...

[1] Lakes impounded by moraines may be considered hazardous in glaciated areas throughout the world because dams can fail suddenly producing destructive floods with peak discharges far in excess of normal flows. Here we present a comprehensive case study in the Zurich, Switzerland, area that reveals several independent lines of evidences for the occurrence of a Late Pleistocene ( 13,760 calibra...

New field evidence and discharge calculation procedures provide new estimates of maximum late Pleistocene glacial Lake Missoula flood discharges for two important reaches along the flood route. Within the Spokane Valley, near the point of release, the peak discharge probably exceeded 17 ± 3 million m-sec. Downstream at Wallula Gap, a major point of flow convergence, peak discharge was about 10 ...

An improved form of a recently derived energetic-statistical formula for size effect on the strength of quasibrittle structures failing at crack initiation is presented and exploited to perform stochastic structural analysis without the burden of stochastic nonlinear finite-element simulations. The characteristic length for the statistical term in this formula is deduced by considering the limi...

The DNA of Serratia marcescens has N6-adenine methylation in GATC sequences. Among 2-aminopurine-sensitive mutants isolated from S. marcescens Sr41, one was identified which lacked GATC methylation. The mutant showed up to 30-fold increased spontaneous mutability and enhanced mutability after treatment with 2-aminopurine, ethyl methanesulfonate, or UV light. The gene (dam) coding for the adenin...

The objective of this study was to evaluate the opinions and attitudes of dentists toward the use of rubber dam during various restorative procedures in their dental practices. A structured questionnaire was designed, and distributed to 300 dentists and those that were fully completed were collected for analysis. The collected informations about the opinions and attitudes of dentists toward the...

Abrupt environmental pollution accidents cause considerable damage worldwide to the ecological environment, human health, and property. The concept of acceptable risk aims to answer whether or not a given environmental pollution risk exceeds a societally determined criterion. This paper presents a case study on acceptable environmental pollution risk conducted through a questionnaire survey car...