AAA+ proteins (ATPases associated with various cellular activities) are oligomeric ATPases that use ATP hydrolysis to remodel their substrates. By similarity with GTPases, a dynamic organization of the nucleotide-binding pockets between ATPase protomers is proposed to regulate functionality. Using the transcription activator PspF as an AAA+ model, we investigated contributions of conserved resi...

Glutamic acid is an important amino that used widely in the fields of food, medicine, and agriculture. One methods glutamic production direct microbial fermentation, so genetic stability glutamic-acid-producing capacity producing strain are keys to improving concentration. Experiments were carried out using Corynebacterium glutamicum GL−6 as parental strain, with two iterations mutagenesis by a...

on the soluble peptide from B. 8ubtili8 by Bovarnick (1942), the B. anthraci8 capsule by Hanby & Rydon (1946) and the soluble peptide from B. meoentericus by Pongor (1950) has shown that, to the extent of at least 99 %, glutamic acid is the only nitrogen-containing substance in the peptide hydrolysates. The optical configuration of the glutamic acid appears to vary from one preparation to anoth...

UNLABELLED Latent autoimmune diabetes in adults (LADA) is a relatively new type of diabetes with a clinical phenotype of type 2 diabetes (T2D) and an immunological milieu characterized by high titers of islet autoantibodies, resembling the immunological profile of type 1 diabetes (T1D). Herein, we report a case of a young male, diagnosed with LADA based on both clinical presentation and positiv...

The possibi]ity of producing amino acids under cenditions of denitrification, i.e., with nitrate respiration as an energy-supplying system, was inyestigated using denitrifying bacteria. We found that Bacirrus 1tehenijbrmis A35 accurnulated 7-polyglutamic acid (PGA) to a concentratien ef8 mg per ml in a medium containing glllcose and ammonium chloride or glutamic acid under denitrifying conditio...

Observations in this laboratory have indicated that human subjects with malignant growths exhibit a significant elevation in plasma glutamic acid concentration (2, 5) . No such elevation was observed in subjects with benign growths, al though it has been shown that an elevated plasma glutamic acid level may be observed in clinical conditions other than malignancy (5). The results suggest that p...

OBJECTIVES Free glutamic acid has an appetite-regulating effect and studies with infant formula have suggested that free amino acids (FAA), especially glutamic acid, can downregulate intake. The content of glutamic acid and glutamine is high in breast milk but varies considerably between mothers. The aim was to investigate whether maternal anthropometry was associated with the content of the FA...

the pb2 protein of influenza a viruses has been shown as host range determinant. in this study pb2gene of five h9n2 isolates from tehran province during the period of 1998-2001 were partially amplifiedand sequenced to do phylogenetic study. iranian isolates could be classified in eurasian lineage according totheir pb2 gene sequences. the pb2 genes of these isolates are different from those of m...

Response surface methodology (RSM) was employed to study the effects of four medium components (L-glutamic acid, citric acid, glycerol and NH4Cl) on the production of poly-γ-glutamic acid (PGA) in flask cultures by Bacillus licheniformis CCRC 12826 (ATCC 9945) with initial medium pH 6.5. The optimal PGA yield (35.34 g/L, average of four repeats) was appeared at the region where the respective c...