In previous investigations imidazoleacetic acid was shown to be quantitatively converted to formylaspartic acid by a partially purified enzyme preparation obtained from Pseudomonas sp. that requires DPNH’ and oxygen (2, 3). An intermediate in this process was isolated from the incubation mixture with highly purified enzyme preparations and was characterized as N-formimino-L-aspartic acid (4). E...

L-Glutamic, L-aspartic acids and a number of their structural analogs, including quisqualic, kainic, ibotenic, quinolinic, and N-methyl-D-aspartic (NMDA) acids, increase inositol phospholipid hydrolysis when added to primary cultures of cerebellar granule cells, as is reflected by an enhanced formation of 3H-inositolmonophosphate (3H-IP1) in the presence of Li+. L-Glutamic acid also enhances th...

Lockhart & Abraham (1956) found that two compounds which appeared to be aspartyl-lysine and aspartyl(isoleucyl)-lysine respectively were formed when bacitracin A was hydrolysed with 11 Nhydrochloric acid at 800. The aspartic acid residue in these compounds had the L-configuration and was linked to the e-amino group of L-lysine. However, both compounds carried a net positive charge at pH 7 and t...

Four kinds of retrovirus protease inhibitors (RPI-856 A, B, C and D) were isolated as white powder from the culture filtrate of a soil isolate, Streptomyces sp. AL-322 by column chromatography using Diaion HP-20, Sephadex LH-20, ODS reversed phase HPLC and SP-2SW ion exchange HPLC. The structures of these inhibitors were elucidated by physico-chemical properties, chemical reactions and spectral...

Minute changes in pH, NaCl concentration Aspartic acid, Glutamic acid and by inference other acids affect the major pathways from acetyl Co-A. The important differences between experiments with and without buffer show the reversal of results. Glutamic acid increases O2 consumption; cholesterol of liver homogenates in media without buffer decreases QO2,. cholesterol and total lipids in media wit...

Objective . To optimize the composition of polymer coatings based on assessment duration antibacterial resistance in infected wounds laboratory animals. Materials and methods Woven vascular prostheses consisting fibrous-porous polypropylene one three types a polyvinyl-chitosan complex with addition biologically active substances were used study. All samples impregnated 1% vancomycin for 30 minu...

We describe a method for making selective lesions of the hippocampus in macaque monkeys, using a magnetic resonance imaging (MRI)-guided stereotaxic approach in which the excitotoxin N-methyl-D-aspartic acid (NMDA) is injected at intervals along a single needle track that extends longitudinally through the rostrocaudal extent of the hippocampus. Procedures were conducted on six rhesus monkeys (...

High D/L aspartic acid ratios are observed in heavy molecular weight aggregates and in water-insoluble protein extracted from whole lenses and nuclear and cortical regions. Purified alpha-, beta-, and gamma-crystallins have low D/L ratios. Fractionation of urea-solubilized material from the water-insoluble protein yields four molecular weight classes of proteins. Fractions representing crosslin...