Human RNA methyltransferase BCDIN3D is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. Here, we show that cytoplasmic tRNAHis is the primary target of BCDIN3D in human cells. Recombinant human BCDIN3D, expressed in Escherichia coli, monomethylates the 5΄-monophosphate of cytoplasmic tRNAHis efficiently in vitro. In BCDN3D-kn...

background- cd24 is a cell adhesion molecule that has been implicated in metastatic tumor progression cells. our aim was clarify correlation between cd24 expression and camp:cgmp ratio in murine colorectal cancer cell line (ct26) after using cholera toxin. materials and methods- the ct26 cells were cultured in microtubes for assaying camp and cgmp; also the cells were cultured in flasks for ass...

Cell-selective regulation of protein translocation is achieved through the rational design an endogenous enzyme-operated spherical nucleic acids platform. The system enables enzyme-activated capture proteins interest and thus their cytoplasmic-nuclear shuttling with tumor-cell specificity, as reported by Mengyuan Li co-workers in Research Article (e202214958).

Antineutrophil cytoplasmic antibodies (ANCA) are predominantly IgG autoantibodies directed against constituents of primary granules of neutrophils and monocytes lysosomes. Although several antigenic targets have been identified, those ANCA directed to proteinase 3 or myeloperoxidase are clinically relevant, whereas the importance of other ANCA remains unknown. Both are strongly associated with ...

In reviewing work on cytoplasmic contractile proteins and the contributions made to this field by The Journal of Cell Biology, some perspective is gained by first pointing out that most cell biologists were not aware of the existence of these important cellular constituents more than 12 years ago. Times have changed, and today cytoplasmic contractile protein research is one of the busiest areas...

The incorporation of tritiated thymidine in Amoeba proteus was reinvestigated in order to see if it could be associated with microscopically detcctable structures. Staining experiments with basic dyes, including the fluorochrome acridine orangc, revealed the presence of large numbers of 0.3 to 0.5 # particles in the cytoplasm of all cells studied. The cffcct of nuclease digestion on the dye aff...

We continue our study of the effects of pollen and seed migration on the cytonuclear structure of mixed-mating plant populations by analyzing two deterministic continent-island models under the critical assumption of paternal cytoplasmic inheritance. The major results of this study that contrast with our previous conclusions based on maternal cytoplasmic inheritance are (i) pollen gene flow can...

A variety of names has been used in the literature for the subunits of cytoplasmic dynein complexes. Thus, there is a strong need for a more definitive consensus statement on nomenclature. This is especially important for mammalian cytoplasmic dyneins, many subunits of which are encoded by multiple genes. We propose names for the mammalian cytoplasmic dynein subunit genes and proteins that refl...

Human genetic studies have identified the neuronal RNA binding protein, Rbfox1, as a candidate gene for autism spectrum disorders. While Rbfox1 functions as a splicing regulator in the nucleus, it is also alternatively spliced to produce cytoplasmic isoforms. To investigate the function of cytoplasmic Rbfox1, we knocked down Rbfox proteins in mouse neurons and rescued with cytoplasmic or nuclea...