Inorganic pyrophosphatase (PPase) is widely studied, to be extremely important for survival of plants and microorganisms. PPases catalyze an essential reaction the hydrolysis of inorganic pyrophosphate (PPi) to inorganic phosphate (Pi). Studies involving the mechanism of PPase were performed in microorganisms culture. We didn’t found reports of PPase derived from soil metagenomic libraries. Soi...

BACKGROUND Jejunal fluid culture is the gold standard for assessing jejunal microflora. Aspiration of jejunal fluid is sometime difficult. As the microorganisms rests on the mucosal surface, culture of the mucosal biopsy may be a possible alternative method. AIM To study the role of jejunal mucosal biopsy culture to assess jejunal microflora and to compare it with jejunal fluid culture. MET...

Bacteria have usually been studied in single culture rich media or under specific starvation conditions. However, nature they coexist with other microorganisms and build an advanced society.

New blood culture media containing antibiotic-binding polymeric beads have been developed for the BacT/Alert (bioMérieux, Inc., Durham, NC) blood culture system. To assess the performance of these new media, we compared the new BacT/Alert aerobic medium (FA Plus) with resins to BacT/Alert FA medium with activated charcoal and the new BacT/Alert anaerobic medium (FN Plus) to BacT/Alert FN medium...

Most bacteria pathogenic to insects are classified in the families Pseudomonadaceae, Enterobacteriaceae, Lactobacillaceae, Micrococcaceae, and Bacilliaceae; spirochetes and rickettsia are in the families Spirochaetaceae and Rickettsiaceae, respectively. Except for Bacilliaceae, these families contain nonsporulating microorganisms. Most spore-forming bacteria pathogenic to insects belong to the ...

Appropriate care for bacteremic patients is dictated by the amount of time needed for an accurate diagnosis. However, the concentration of microbes in the blood is extremely low in these patients (1-100 CFU/mL), traditionally requiring growth (blood culture) or amplification (e.g., PCR) for detection. Current culture-based methods can take a minimum of two days, while faster methods like PCR re...

background and objectives: coagulase negative staphylococcus (cons) is frequently isolated from blood cultures but their significance is difficult to interpret. cons bacteria which are often previously dismissed as culture contaminants are attracting greater importance as true pathogens in the past decades. clinical evaluation of these isolates suggests that although there is a relative increas...