Article history: Received on: 29/04/2013 Revised on: 02/06/2013 Accepted on: 17/06/2013 Available online: 27/06/2013 This study reports the antibacterial activity of coral reef associated bacteria against bacterial pathogens and optimization of metabolite production. Twelve morphologically different bacterial were isolated from stony coral reef collected from Rameshwaram coastal area, Tamil Nad...

AIMS Experiments were designed to detect quorum-sensing signals produced by Clostridium botulinum. METHODS AND RESULTS Clostridium botulinum 56A cell-free supernatants obtained at the end of lag phase, the mid-exponential phase and early stationary phase of growth were assayed for bioluminescence in the Vibrio harveyi quorum-sensing assay system. Twelve and 16-h culture supernatants induced b...

1. Cultures of Diplococcus pneumoniae, Bacillus coli, Bacillus fluorescens liquefaciens and Bacillus prodigiosus, when grown in meat infusion broth exhibit an initial latent period when the culture used for inoculation is no longer growing at its maximum rate; if, however, the culture is growing at its most rapid rate the bacteria, upon subculture, show no latent period but continue to multiply...

Silver nanoparticles have attracted high attention worldwide for their various applications. The physiochemical parameters such as temperature, media, mixing ratio affect the rate of synthesis silver and yield. Thus, optimization these is needed to enhance production nanoparticles. In this study, were synthesized using Aspergillus niger culture supernatant. produced characterized UV-visible Spe...

The extracelluar domain I-IV of target gene VEGFR2 (Vascular endothelial growth factor receptor 2) was cloned from villus of trimester abortion by RT-PCR, and linked to the expression vectors. Then, the transfection conditions were optimized in serum-free suspension culture HEK293 using GFP (Green fluorescence protein) as the report gene. The results showed that the optimal transfection efficie...

objective(s) some properties of neuraminidase produced by pseudomonas aeruginosa pao1 growth in a defined medium (bhi) were examined and evaluated for its features. materials and methods the obtained supernatant enzyme of p. aeruginosa pao1 cultures was used in a sensitive fluorometric assay by using 2'-(4-methylumbelliferyl) a-d-n acetylneuraminic acid as substrate. as hydrolyzing mun wit...

The probiotic Enterococcus faecium HDRsEf1 (Ef1) has been shown to have positive effects on piglet diarrhoea, but the mechanism has not yet been elucidated. In this study, using the IPEC-J2 cell line to mimic intestinal epithelial cells and enterotoxigenic Escherichia coli (ETEC) K88ac as a representative intestinal pathogen, the mechanism underlying Ef1 protection against an enteropathogen was...

Since in several vascular diseases abnormal vascular smooth-muscle cell (VSMC) proliferation is often associated with the presence of macrophages, we examined whether macrophage-colony-stimulating factor (M-CSF) might play a role in the control of VSMC growth. VSMCs were isolated from rat aorta and maintained in culture. Using a bioassay, a macrophage-colony-stimulating activity was detected in...

We developed an efficient cell culture system for genotype 4 hepatitis E virus using the HE-JF5/15F strain recovered from a fulminant hepatitis patient. The sixth-passage virus in the culture supernatant reached 1.5 x 10(8) copies/ml at 10 days postinoculation and possessed 10 nucleotide mutations with four amino acid changes.