introduction: decidua as the nearest mother's tissue layer to the fetus which is in direct contact with the embryo has an important role in composing the appropriate microenvironment for protection of allogenic fetus from mother's immune response. in this study the effect of supernatant from culture of decidual cells on dendritic cells regarding the induction of t-cell allogenic proliferation r...

The culture media optimization is an inevitable part of upstream process development in therapeutic monoclonal antibodies (mAbs) production. The quality by design (QbD) approach defines the assured quality of the final product through the development stage. An important step in QbD is determination of the main quality attributes. During the media optimization, some of the main quality attribute...

most of the systematic studies used in the development of human embryo culture media have been done first on mouse embryos. the general use of nmri outbred mice is a model for toxicology, teratology and pharmacology. nmri mouse embryo exhibit the two-cell block in vitro. the objective of this study was to evaluate and compare the effects of four kinds of culture media on the development of zygo...

introduction: the purpose of this study was to cultivate bone marrow cells from two different mouse strains (nmri and balb/c) and to examine them in terms of expression of ten surface antigens from primary culture to passage 3. material and methods: 6-8 weeks old either nmri or balb/c mice were sacrificed and their bone marrows were harvested and cultivated .two weeks after culture initiation, ...

It is now more than 50 years since Ross Harrison (1907) inaugurated the technique of tissue culture in vitro, and at the same time settled for ever the controversy as to the origin of nerve fibres; he explanted fragments of a tadpole's spinal cord in a drop of lymph spread on a coverslip sealed to a hollow-ground slide and observed the outgrowth of naked nerve fibres into the surrounding fluid....

At noon on September 18 th , 2010, the mayor of Munich will kick off Oktoberfest with the traditional ''O'zapft is'' as he taps the first keg of the festival. Over the course of the next 2 weeks, more than six million people will celebrate the 200 th anniversary of Oktoberfest by savoring roasted chicken, juicy weisswurst, and giant pretzels. But, most importantly, there will be beer—enough bee...

Numerous studies have shown the positive effects of rosmarinic acid on the nervous system. Rosmarinic acid as a herbal compound with anti-inflammatory effects can prevent the destructive effect of inflammation on the nervous system. Furthermore, various studies have emphasized the advantages of three dimensional (3D) culture over the two dimensional (2D) culture of cells. In this study, thermos...

Cell Culture Bioreactors Basic Types of Bioreactors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 Stirred Tank (Well Mixed) vs. Tubular Reactor (Plug Flow) . . . . . . . . . . . . . . . . . 3 Segregated Bioreactors (Dead Zone Present) Compartmentalized Bioreactors . . 4 Implication When Growth or Reaction Occurs in the Reactor . . . . . ....

In vivo cell culture has been known for several years. The earliest attempts were made by Rezzesi (18) and Bisceglie (6). These investigators reported that Ehrlich carcinoma of mice survived for 12 days in the peritoneal cavity of guinea pigs when cultured in collodion dialysis sacs. More recently Prehn, Weaver, and Algire (16, 17) introduced the diffusion chamber technic using Millipore Filter...

organotypic spinal cord slices from neonatal mammals could be a powerful model for evaluation of cell survival but also cell death mechanisms. the aim of this study was to establish an in vitro model for investigating cell survival and mechanism involved in cell death in neonatal spinal cord slices. the spinal cord was sliced and incubated into culture medium. the mtt assay was carried out to a...