The overall design of an industrial crop production system should optimize the system's global parameters, such as total area required, intensity of cultivation and general production schedule. The key element is the optimal intensity of cultivation for the prevailing climatic and economic environments. A single-state-variable vegetative crop model (e.g. lettuce) was used to optimize a crop pro...

Process conditions for the synthesis and subsequent secretion of ectoine and hydroxyectoine were optimized in a cascade of two continuously operated bioreactors. In the first bioreactor the halophile bacterium Chromohalobacter salexigens was grown under continuous hyperosmotic and thermophilic conditions and therefore under constant stress driving the cells to accumulate large amounts of ectoin...

Several reviews have analyzed the factors that affect the change in soil organic C (SOC) when forest is converted to agricultural land; however, the effects of forest type and cultivation stage on these changes have generally been overlooked. We collated observations from 453 paired or chronosequential sites where forests have been converted to agricultural land and then assessed the effects of...

In order to assess the physiological responses of bradyrhizobia and competition for the nodulation of soybean at different temperatures, we investigated the expression of the nodC gene at 20, 25, and 30°C and the abilities of bacteria to nodulate soybean in microcosms at day/night cultivation temperatures of 23/18°C, 28/23°C, and 33/28°C for 16/8 h. We tested five Bradyrhizobium USDA strains: B...

climate is one of the most important factors that influence the quality and quantity of crops. understanding the natural features of any region, especially about the climate, can play a major role in making plans and land use planning. sugar beet, is one of the major industrial plants, that in addition to producing one of the most fundamental basic needs of society, namely sugar, it can have ot...

In this study, we compared basic expression approaches for the efficient expression of bioactive recombinant human interleukin-6 (IL6), as an example for a difficult-to-express protein. We tested these approaches in a laboratory scale in order to pioneer the commercial production of this protein in Escherichia coli (E. coli). Among the various strategies, which were tested under Research and De...

The influence of different nitrate concentrations in combination with three cultivation temperatures on the total fatty acids (TFA) and eicosapentaenoic acid (EPA) content of Nannochloropsis salina was investigated. This was done by virtue of turbidostatic controlled cultures. This control mode enables the cultivation of microalgae under defined conditions and, therefore, the influence of singl...

Fibropapillomatosis (FP) of green turtles has a global distribution and causes debilitating tumours of the skin and internal organs in several species of marine turtles. FP is associated with a presently non-cultivable alphaherpesvirus Chelonid fibropapilloma-associated herpesvirus (CFPHV). Our aims were to employ quantitative PCR targeted to pol DNA of CFPHV to determine (i) if DNA sequesters ...

The ability of human clinical isolates of Yersinia enterocolitica to interact with HEp-2 cells was found to vary considerably between the serotypes O:3, O:8, and O:9. Although all three serotypes adhered initially to the cell surface, regardless of incubation temperature of the bacterial inoculum or presence of the 40- to 48-megadalton virulence plasmid, the ability to localize intracellularly ...

BACKGROUND The yeast Pichia pastoris (P. pastoris) has become a popular 'cell factory' for producing heterologous proteins, but production widely varies among proteins. Cultivation temperature is frequently reported to significantly affect protein production; however, the underlying mechanisms of this effect remain unclear. RESULTS A P. pastoris strain expressing recombinant human interleukin...