Using a technique similar to that devised by Rivers (1) for the cultivation of vaccinia virus, Haagen and Tbeiler (2) reported successful cultivation of a strain of yellow fever virus which had been greatly modified through many mouse passages by intracerebral inoculation. The medium consisted of chick embryo tissue and Tyrode solution containing 10 per cent of normal monkey serum. However, eff...

A common practice on Israeli dairy barns comprises daily cultivation of the manure. Cultivation is a mechanical process used to break up and till the manure bedding and it results in a drier and aerated bedding and cleaner cows, which consequently reduces the incidence of mastitis. Cultivation was associated with a shorter survival of Escherichia coli in cultivated manure as compared with noncu...

Using a system of whole-embryo culture, the effect of polybrominated biphenyls (PBB) was investigated on the rates of DNA, RNA, and protein accumulation in rat embryos. Embryos were isolated on the 10th gestational day (sperm day = Day 0) either 4 or 24 hr after maternal administration. The cultivation period was for 24 or 42 hr. DNA, RNA, and protein accumulation rates were not affected by a 4...

Polyhydroxybutyrate (PHB) is one of the polyhydroxyalkanoates (PHAs) which has biodegradable and biocompatible properties. They are adopted in the biomedical field, in, for example, medical implants and drug delivery carriers. This study seeks to promote the production of PHB by Vibrio sp. BM-1, isolated from a marine environment by improving constituents of medium and implementing an appropria...

Title of Thesis: THATCH AND SOIL PESTICIDE DEGRADATION AND MICROBIAL ACTIVITY AS INFLUENCED BY TURF CULTIVATION PRACTICES Yusong Mu, Master of Science, 2009 Thesis Directed By: Mark J. Carroll, Ph.D. Associate Professor Department of Plant Science and Landscape Architecture Pesticide degradation in turf is complicated by presence of an organic matter enriched layer called thatch. It is not well...

Animals modify behavioral outputs in response to environmental changes. C. elegans exhibits thermotaxis, where well-fed animals show attraction to their cultivation temperature on a thermal gradient without food. We show here that feeding-state-dependent modulation of thermotaxis is a powerful behavioral paradigm for elucidating the mechanism underlying neural plasticity, learning, and memory i...

A simple method for plasmid minipreps in closed 1.5 mL microcentrifuge tubes using a cultivation medium with internal substrate delivery (EnBase(®)) in combination with a two-phase perfluorodecalin (PFD) system supplying additional oxygen to the E. coli culture is described. The procedure can simply be performed on a thermoshaker using only 50 μL cultivation volume. Twenty and twenty-five perce...

Some months ago there was published from this laboratory a brief preliminary report of a method whereby tumors could be cultivated in the yolk sacs of developing chick embryos (4). Neoplasms were first injected into the yolk sac with the idea that the tumor cells would die and disintegrate, thus releasing any virus that might be present. It was hoped that if such a release occurred the medium p...

The immediate effects of tillage on protected soil C and N pools and on trace gas emissions from soils at precultivation levels of native C remain largely unknown. We measured the response to cultivation of CO2 and N2O emissions and associated environmental factors in a previously uncultivated U.S. Midwest Alfisol with C concentrations that were indistinguishable from those in adjacent late suc...

BACKGROUND The 'attached cultivation' technique for microalgae production, combining the immobilized biofilm technology with proper light dilution strategies, has shown improved biomass production and photosynthetic efficiency over conventional open-pond suspended cultures. However, how light is transferred and distributed inside the biofilm has not been clearly defined yet. RESULTS In this r...