The immunogold-silver staining (IGSS) technique in combination with epi-fluorescence detection was used to localise cucumber mosaic virus (CMV) particles within banana infected tissues. For this purpose, tissue samples (2 mm3) were excised from CMV-infected and highly proliferating meristem cultures of Williams BSJ banana (ITC. 0570, AAA, Cavendish subgroup). These samples were immediately fixe...

The nucleotide sequence of RNA 1 of the Fny strain (Subgroup I) of cucumber mosaic virus (CMV) was determined and compared at both the nucleic acid and protein levels with the corresponding sequence of RNA 1 of the Q strain (Subgroup II) of CMV. Fny-CMV RNA 1 consisted of 3357 nucleotides and contained a single long open reading frame (ORF) of 2979 nucleotides, whereas Q-CMV RNA 1 consists of 3...

Background: Cucumber mosaic virus (CMV) from the Bromoviridae family, is one of the most widespread plant viruses in the world. Objectives: In the present study tomato fields in Guilan, Isfahan, Khorasan Razavi, Khuzestan and Tehran provinces were surveyed to determine the presence of CMV subgroups during 2011-2012. Materials and Methods: Out of 305 symptomatic leaf samples ...

The nucleotide sequence of RNA 3 of two subgroup I strains of cucumber mosaic virus (CMV), Fny-CMV and M-CMV, was determined and compared at both the nucleic acid and protein level with the previously determined, corresponding (partial) sequences of RNA 3 of five other subgroup I strains: C-CMV, D-CMV, I17F-CMV, O-CMV and Y-CMV. Fny-CMV RNA 3 is composed of 2216 nucleotides (nt) and M-CMV RNA 3...

Cucumber mosaic virus (CMV) is an RNA plant virus with a tripartite genome and an extremely broad host range. Previous evolutionary analyses with the coat protein (CP) and 5' nontranslated region (NTR) of RNA 3 suggested subdivision of the virus into three groups, subgroups IA, IB, and II. In this study 15 strains of CMV whose nucleotide sequences have been determined were used for a complete p...

Cucumber mosaic virus (CMV) is an isometric virus which infects a wide range of hosts in the temperate regions of the world (Kaper & Waterworth, 1981). CMV genomic R N A s consist of four components (mol. wt. 1.09, 0-98, 0.71 and 0.33, all x 106). The three largest components carry all the information required for virus replication, whereas the fourth component corresponds to the part of the ge...

Cucumber mosaic virus (CMV) is the type species of the Cucumovirus genus in the Family Bromoviridae. The virus is distributed worldwide, has a very broad host range infecting over 1000 species in more than 85 plant families, and is transmitted by more than 80 aphid species in 30 genera in a non-persistent manner. The viral genome is positive-sense, single-stranded RNA divided among three segmen...