Background Whole ovary cryopreservation has been proved to be a feasible technique in fertility preservation. The normal ovarian cortex contains a large population of primordial follicles, this characteristic make them far more tolerant to cryoinjury. Researchers have used different cryoprotectants and various techniques to improve cryopreservation. It is plausible that inclusion of an antioxid...

C urrent guidelines recommend antral isolation of the pulmonary veins (PV) as the cornerstone of atrial fibrillation ablation. 1 Although conventional radiofrequency energy has traditionally been used with a point-by-point approach, cryo-ablation has emerged as a viable alternative to radiofrequency energy. The clinical success of cryoablation has been driven by the introduction of the cryoball...

The aims of the present study were to determine the number of spermatozoa in the reproductive tract of sows after intra-uterine insemination (IUI) with frozen-thawed (FT) boar semen and to investigate the influence of adding seminal plasma in the thawing medium on the sperm transport in the female reproductive tract. Fourteen multiparous sows were divided into 3 groups: group I (n= 5), insemina...

Light Source INTRODUCTION This study presents a new device prototype for visualization of physical effects associated with large-scale cryopreservation—the preservation of tissues at very low temperatures. Cryopreservation represents the only method for long-term preservation of biomaterials. While techniques for cryopreservation of single cells and small tissue structures are well established,...

The regenerative capacity of adult human hearts after infarction seems vestigial at best, perhaps because of the poor proliferative capacity of terminally differentiated cardiomyocytes and desecration of the local environment that would otherwise be conducive of stem cellmediated repair or loss of stem cells, or both. In a recent edition of the journal Science, Porello et al set a new benchmark...

compared with 201Tl SPECT imaging. Radiology 2004;232:49–57. 24. Asanuma T, Tanabe K, Ochiai K, Yoshitomi H, Nakamura K, Murakami Y, Sano K, Shimada T, Murakami R, Morioka S, Beppu S. Relationship between progressive microvascular damage and intramyocardial hemorrhage in patients with reperfused anterior myocardial infarction: myocardial contrast echocardiographic study. Circulation 1997;96: 44...

Heparin binding proteins (HBPs) are produced by accessory glands. These are secreted into the seminal fluid, bind to the spermatozoa at the time of ejaculation, favour capacitation, acrosome reaction, and alter the immune system response toward the sperm. The present study was conducted with an objective to assess the effect of purified seminal plasma-HBPs (SP-HBPs) on cross bred cattle bull sp...

Abstract Study question Is the human egg spindle integrity impaired by vitrification and thawing procedures? Summary answer Cryoprotective-caused dehydration stabilizes MII spindle, but re-entry of water during induces depolymerization microtubules, thus promoting instability division apparatus. What is known already Freezing sperm preimplantation embryos have become routine IVF procedures with...

Abstract Study question Does cryopreservation impact on transcriptome of conventional frozen human spermatozoa? Summary answer The sperm alters pathways important for fertility, although the upregulation some genes can compensate harmful effects freezing. What is known already Sperm a widely used procedure storing gametes later use, preserving fertility in patients before gonadotoxic treatments...

A new methodology to control the freezing front propagation during cryosurgical procedures is studied through the use of numerical techniques. Laser irradiation of a target tissue is explored as a new methodology for localizing heat generation and, thus, confining more accurately the desired cryoinjury region and to protect a thicker superficial layer of tissue. In addition to the irradiation o...