Porphyrias are the group of orphan diseases, related to pathological disruptions heme biosynthesis. These diseases hard diagnose timely and existing methods lack universality. High performance liquid chromatography coupled with ultraviolet detector could be adapted for this purpose via determination porphyrins in human urine. Waters Acquity UPLC H-CLASS diode matrix was used study. Developed me...

Identification and quantitative determination methods of arsenic species in environmental and biological samples are discussed. Isolation followed by molecular spectroscopic determination is necessary for rigorous identification while element-specific detections coupled with separation techniques are the choice for quantitative determination. Analytical figures of merit are given for the method...

Comprehensive two-dimensional liquid chromatography (LC×LC) using the Agilent 1290 Infi nity 2D-LC Solution with DAD and MS detection is applied for the analysis of citrus oils. The developed method uses normal phase liquid chromatography in the fi rst dimension and reversed-phase liquid chromatography in the second dimension. This combination provides good orthogonality and is valuable for pro...

This review describes the role of wood extractives, especially fatty and resin acids, in papermaking, as well as the importance of their removal from process waters. One of the main aims is also to illustrate versatile analysis methods for this purpose and highlight recent developments in corresponding applications. Most of the current methods require timeconsuming and laborious sample pretreat...

Analysis of catecholamines (epinephrine, norepinephrine and dopamine) in plasma and urine is used for diagnosis and treatment of catecholamine-producing tumors. Current analytical techniques for catecholamine quantification are laborious, time-consuming and technically demanding. Our aim was to develop an automated on-line solid phase extraction method coupled to high performance liquid chromat...

In recent years there has been considerable interest in the synthesis and separation of enantiomers of organic compounds especially because of their importance in the biochemistry and pharmaceutical industry. High-performance liquid Chromatography is a very useful method for the direct separation of enantiomers. However, about 30−40 years ago, commercially available chiral stationary phases wer...