نتایج جستجو برای: coronavirus sars cov 2

تعداد نتایج: 2561448  

2010
Aartjan J. W. te Velthuis Sjoerd H. E. van den Worm Amy C. Sims Ralph S. Baric Eric J. Snijder Martijn J. van Hemert

Increasing the intracellular Zn(2+) concentration with zinc-ionophores like pyrithione (PT) can efficiently impair the replication of a variety of RNA viruses, including poliovirus and influenza virus. For some viruses this effect has been attributed to interference with viral polyprotein processing. In this study we demonstrate that the combination of Zn(2+) and PT at low concentrations (2 µM ...

Journal: :Journal of molecular biology 2007
Xiaoyu Xue Haitao Yang Wei Shen Qi Zhao Jun Li Kailin Yang Cheng Chen Yinghua Jin Mark Bartlam Zihe Rao

The viral proteases have proven to be the most selective and useful for removing the fusion tags in fusion protein expression systems. As a key enzyme in the viral life-cycle, the main protease (M(pro)) is most attractive for drug design targeting the SARS coronavirus (SARS-CoV), the etiological agent responsible for the outbreak of severe acute respiratory syndrome (SARS) in 2003. In this stud...

Journal: :Journal of clinical microbiology 2004
Liqun Lu Ivanus Manopo Bernard P Leung Hiok Hee Chng Ai Ee Ling Li Lian Chee Eng Eong Ooi Shzu-Wei Chan Jimmy Kwang

Severe acute respiratory syndrome (SARS) is a novel infectious disease caused by the SARS-associated coronavirus (SARS-CoV). There are four major structural proteins in the SARS-CoV, including the nucleocapsid, spike, membrane, and small envelope proteins. In this study, two sets of truncated fragments of spike protein were generated, the first were approximately 210-bp nonoverlapping fragments...

Journal: :Journal of Biomedicine and Biotechnology 2008
K.-Y. Hwa W. M. Lin Y.-I. Hou T.-M. Yeh

Molecular mimicry, defined as similar structures shared by molecules from dissimilar genes or proteins, is a general strategy used by pathogens to infect host cells. Severe acute respiratory syndrome (SARS) is a new human respiratory infectious disease caused by SARS coronavirus (SARS-CoV). The spike (S) protein of SARS-CoV plays an important role in the virus entry into a cell. In this study, ...

Journal: :Journal of virology 2011
Stephanie Bertram Ilona Glowacka Marcel A Müller Hayley Lavender Kerstin Gnirss Inga Nehlmeier Daniela Niemeyer Yuxian He Graham Simmons Christian Drosten Elizabeth J Soilleux Olaf Jahn Imke Steffen Stefan Pöhlmann

The highly pathogenic severe acute respiratory syndrome coronavirus (SARS-CoV) poses a constant threat to human health. The viral spike protein (SARS-S) mediates host cell entry and is a potential target for antiviral intervention. Activation of SARS-S by host cell proteases is essential for SARS-CoV infectivity but remains incompletely understood. Here, we analyzed the role of the type II tran...

Background and purpose: Coronavirus disease 2019 (COVID-19) is a respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, an indirect ELISA method was designed to measure the human IgM and IgG antibodies against SARS-CoV-2. Materials and methods: Protein sequence of nucleocapsid antigen from SARS-CoV-2 was expressed in E. coli BL21 and then was...

2012
Changhyun Roh

Hundreds of million people worldwide have been infected with severe acute respiratory syndrome (SARS), and the rate of global death from SARS has remarkably increased. Hence, the development of efficient drug treatments for the biological effects of SARS is highly needed. We have previously shown that quantum dots (QDs)-conjugated RNA oligonucleotide is sensitive to the specific recognition of ...

Journal: :Bioorganic & medicinal chemistry 2004
Xue Wu Zhang Yee Leng Yap

The main proteinase of SARS-associated coronavirus (SARS-CoV) plays an important role in viral transcription and replication, and is an attractive target for anti-SARS drug development. The important thing is to understand its binding mechanism with possible ligands. In this study, we investigated possible noncanonical interactions, potential inhibitors, and binding pockets in the main proteina...

2004
Guodong Liang Qiuxia Chen Jianguo Xu Yufei Liu Wilina Lim J.S.M. Peiris Larry J. Anderson Li Ruan Hui Li Biao Kan Biao Di Peter Cheng K.H. Chan Dean D. Erdman Shuyan Gu Xinge Yan Weili Liang Duanhua Zhou Lia Haynes Shumin Duan Xin Zhang Han Zheng Yang Gao Suxiang Tong Dexin Li Ling Fang Pengzhe Qin Wenbo Xu

Four cases of severe acute respiratory syndrome (SARS) that occurred from December 16, 2003, to January 8, 2004, in the city of Guangzhou, Guangdong Province, China, were investigated. Clinical specimens collected from these patients were tested by provincial and national laboratories in China as well as members of the World Health Organization SARS Reference and Verification Laboratory Network...

Journal: :iranian journal of virology 0
helya sadat mortazavi iran university of medical sciences seyed hamidreza monavari iran university of medical sciences angila ataei pirkooh iran university of medical sciences ahmad tavakoli iran university of medical sciences

the recently emerged middle east respiratory syndrome coronavirus (mers-cov) emerged in the middle east region in 2012. the virus is phylogenetically related to bat cov, but other animal species like camels and goats may potentially act as an intermediate host by spreading the virus to humans. this virus is thought to cause a severe disease in patients with underlying comorbidities. laboratory ...

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