The performance of the Virogen Rotatest latex agglutination test (LAT) was evaluated for detection of bovine rotavirus antigen. Sixty-three fecal samples from diarrheic calves were collected from November 1999 to May 2000 and screened by LAT, the Rotazyme II enzyme-linked immunosorbent assay (ELISA), and virus isolation (VI) followed by an anti-rotavirus fluorescent-antibody (FA) test to detect...

In the reference laboratory complete serological identification of salmonella requires the identification of 59 'O' (somatic) and 87 'H' (flagellar) antigens. When large numbers of strains are studied mechanisation of the serological techniques would be advantageous. Normally, slide and tube agglutination methods are used,' 2 and the latter is an essential confirmatory test. The antisera used a...

A total of 163 stool specimens were tested for detection of Clostridium difficile and its toxin by cytotoxicity assay with tissue culture, latex agglutination test, and isolation of the organism. From 33 specimens which were positive for toxin by cytotoxicity, 30 were positive by the latex agglutination test; the organism was isolated from 21. The total number of samples which were positive wit...

The purpose of this study was to survey infections with Salmonella spp. in poultry flocks in the vicinity of Tehran and to determine the most frequent serogroups and serotypes implicated. Twenty-eight samples of pullet, layer, and broiler flocks were randomly collected (n=1463), including freshly dropped feces from live birds or visceral organs from dead birds. In most flocks, 60 samples were t...

Anemic patients often have low hemoglobin and red blood cell level, which on the slide method ABO system type test is used to obtain degree of agglutination. Addition treatment a centrifugation was stronger The purpose this research determine difference agglutination between centrifuged uncentrifuged specimens in anemic patients. Quasi experimental with non-equivalent control group design condu...