dCMP deaminase was partially purified from BHK-21/C13 cells grown in culture. The molecular weight of the enzyme was estimated by gel filtration and gradient centrifugation to be 130000 and 115000 respectively. The enzyme had a pH optimum of 8.4. Its activity versus substrate concentration curve was sigmoid, the substrate concentration at half-maximal velocity being 4.4mm. dCTP activated the de...

The aim of the present investigation was to assess neuroprotection exerted by GABA (100 nM-100 mM) in rat cortical brain slices subjected to oxygen-glucose deprivation and reoxygenation. Neuronal injury and neuroprotection were assessed by measuring the release of glutamate and lactate dehydrogenase and tissue water content. Results demonstrate that GABA exerted neuroprotective effects accordin...

In an attempt to detect an increased bronchial blood flow in man from the earpiece dye-dilution curve, the reliability of three indexes was investigated by comparing them with the values of bronchial blood flow measured from the left atrial dye-dilution curve following dye injection into the aortic root. The first index is obtained from the Cp method, in which the ratio of the peak concentratio...

Area-Under-the-Concentration Curve, Bruce Kaplan,* Herwig-Ulf Meier-Kriesche, Kimberly Napoli, and Barry D. Kahan (Div. of Renal Dis. and Hypertension, Dept. of Internal Med., and Div. of Immunol. and Organ Transplant., Dept. of Surgery, The University of Texas Medical School at Houston, 6431 Fannin, Suite 4.163, Houston, TX 77030; *author for correspondence and reprint requests: fax 713-794-1197)

H. Tsuruta1; M. Fukumoto2; L. Bax3; A. Kohno4; Y. Morishita4 1Department of Medical Informatics, School of Allied Health Sciences, Kitasato University, Kanagawa, Japan; 2Division of Toxicology, Center for Clinical Pharmacy and Clinical Sciences, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan; 3Pharsight Corporation, Sunnyvale, CA, USA; 4Department of Hematology and Oncolog...

Antitumor activity of topotecan, a new derivative of camptothecin, has been documented in many tumors. The active lactone form of topotecan is in equilibrium with the inactive hydroxyacid form. However, dose-limiting toxicity, neutropenia, is correlated to the area under the concentration time curve (AUC) of not only the lactone form but also total (lactone + hydroxyacid) topotecan. Because the...

Background: Selective phosphodiesterase (PDE3) inhibitors improve cardiac contractility and may use in congestive heart failure. However, their proarrhythmic potential is the most important side effect. Methods: In this research, we evaluated the potential cardiotonic activity of six new synthesized selective PDE3 inhibitors (6-hydroxy-4-methylquinolin-2(1H)-one derivatives) using the spontan...

The distribution of income related health inequalities appears to exhibit changing patterns when both developing countries and developed countries are examined. This paper tests for the existence of a health Kuznets' curve; that is, an inverse U-shape pattern between economic developments (as measured by GDP per capita) and income-related health inequalities (as measured by concentration indice...