BACKGROUND Esophageal Squamous Cell Carcinoma (ESCC) is a major subtype of esophageal cancer causing significant morbility and mortality in Asia. Mechanism of initiation and progression of this disease is unclear. Tumor initiating cells (TICs) are the subpopulation of cells which have the ability to self-renew, as well as, to drive initiation and progression of cancer. Increasing evidence has s...

The increase of eukaryotic translation initiation factor 4E (eIF4E) expression is frequently observed in several types of cancer, making eIF4E an attractive anticancer drug target. However, the role of eIF4E in gastric cancer pathogenesis remains unclear. Perifosine is a bioavailable alkylphospholipid exhibiting antitumor activity in a series of cancer types. In this study, gastric cancer cell ...

We have recently described an assay system for human peripheral blood megakaryocyte colony-forming unit cells (CFU-M) using an anti-platelet glycoprotein antiserum probe to define megakaryocyte colonies grown in vitro. This system was applied to study the nature and regulation of human bone marrow CFU-M. In the absence of a specific megakaryocyte growth-promoting factor, 12.4 +/- 3.0 (means +/-...

Three new sulfated steroid monoglycosides, leptaochotensosides A-C (1-3), and a new sulfated polyhydroxylated steroid (4) were isolated from the alcoholic extract of the Far Eastern starfish Leptasterias ochotensis. The structures of compounds 1-4 were established by extensive nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESIMS) analyses and chemical transforma...

Purpose: To elucidate the role of AKAP12 in leukemia cells.Methods: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting (WB) were employed to determine expression leukocyte cell lines, while 5-azacytidine was used treat cells, followed by assessment AKAP12. After constructing overexpressing vector pc-AKAP12 transfecting it into cells or treating with 5-az...

Abstract Background The long noncoding RNA gastric cancer associated transcript 3 (GACAT3) has been demonstrated to be implicated in the carcinogenesis and progression of many malignancies. However, GACAT3’s levels role esophageal squamous cell carcinoma (ESCC) not elucidated. Methods GACAT3 amounts were investigated ESCC tissues lines by qPCR. Its biological functions examined CCK-8 assay, col...

Abstract Background Endometrial cancer is an invasive gynecological prevalent in the world. The pathogenesis of endometrial related to multiple levels regulation, referring oestrogen, tumor-suppressor gene (e.g. PTEN ) or microRNAs miR-23a and miR-29b). Metapristone a hormone-related drug, which widely used clinical treatment cancer. However, underlying regulatory mechanism metapristone on stil...

Radioresistance is an important cause of cancer treatment failure. Circular RNAs (circRNAs) play crucial roles in development, including the radioresistance. This research aimed to determine function and related mechanism circ_0086720 radioresistance non-small cell lung (NSCLC). The expression circ_0086720, miR-375, Spindlin 1 (SPIN1) was measured using a quantitative real-time polymerase chain...

Fish/Radiosensitivity/Colony formation/Mutagens The sensitivities of three goldfish-cell lines to the lethal effects of four carcinogens/mutagens; 4-nitro quinoline-1-oxide (4NQO), 1-methyl-3-nitro-l-nitrosoguanidine (MNNG), mitomycin C (MMC) and bleo mycin (BLM), were compared using colony-formation assay. RBCF-1 and CAF-MM1 cells were more sensitive to 4NQO, BLM and UV than were GEM 199 cells...