نتایج جستجو برای: colony assay

تعداد نتایج: 273197  

2015
Mohammad Hossein Abnosi Elham Shojafar

OBJECTIVES In previous investigations, we have shown para-nonylphenol (p-NP) caused significant reduction of proliferation and differentiation of rat bone marrow mesenchymal stem cells (MSCs) in vitro. In this study, we first treat the rats with p-NP, then carried out the biochemical and morphological studies on MSCs. MATERIALS AND METHODS Proliferation property of cells was evaluated with th...

Journal: :Phytopathology 2002
Blanca B Landa Henricus A E de Werd Brian B McSpadden Gardener David M Weller

ABSTRACT Pseudomonas fluorescens strains producing the antibiotic 2,4-diacetylphloroglucinol (DAPG) have biocontrol activity against a broad spectrum of root and seedling diseases. In this study, we determined the effect of genotype on the ability to isolate and quantify introduced 2,4-DAPG producers from the rhizosphere of wheat using three different methods: traditional dilution plating on se...

2016
Yu-Huai Ho Lih-Shinn Wang Hui-Li Jiang Chih-Hui Chang Chia-Jung Hsieh Dan-Chi Chang Hsin-Yu Tu Tan-Yun Chiu Huei-Jen Chao Chun-Chieh Tseng

Contaminated surfaces play an important role in the transmission of pathogens. We sought to establish a criterion that could indicate "cleanliness" using a sampling area-adjusted adenosine triphosphate (ATP) assay. In the first phase of the study, target surfaces were selected for swab sampling before and after daily cleaning; then, an aerobic colony count (ACC) plate assay of bacteria and anti...

Journal: :Cancer research 1981
E C Bradley F W Ruscetti

A variety of solid and hematological human tumors and normal human bone marrow specimens were assayed for colony formation in a short-term soft-agar culture system. The effect of human fibroblast, lymphoid, and myeloid interferons on inhibition of colony formation was assessed. The effect of interferon on colony formation formed a continuum from complete inhibition to stimulation of growth. Of ...

2017
Jing Dong Zhi Zhang Tao Gu Shu-Feng Xu Li-Xin Dong Xin Li Bao-Hong Fu Zhan-Zhao Fu

OBJECTIVE This study aimed at exploring the role of microRNA-21 (miR-21) in predicting brain metastases (BM) from non-small cell lung cancer (NSCLC). METHODS A total of 132 NSCLC patients, including 68 patients with BM and 64 patients without BM, were included in the study. NSCLC cells were collected and assigned to the inhibitor (IN) group, the mock group, and the negative control (NC) group...

Journal: :Oncology reports 2008
Hideya Yamazaki Ken Yoshida Yasuo Yoshioka Fumiaki Isohashi Shuji Ozeki Masahiko Koizumi Makoto Yoneda Takehiro Inoue

The purpose of this study was to evaluate the impact of mitochondrial DNA (mtDNA) on radiation sensitivity under hypoxic conditions. The cell lines used were rho+ and rho0, which carry wild-type mtDNA and no mtDNA, respectively. The rho0 cells do not utilize oxygen because they lack the capacity to carry out oxidative phosphorylation. To confirm the role played by mtDNA in different cell lines,...

Journal: :International journal of molecular medicine 2010
Xiao-Sheng Hu Chang-Qing Du Lin Yang Xue-Yan Yao Shen-Jiang Hu

The aim of this study was to determine whether proteasome inhibitor MG132 treatment has any effect on endothelial progenitor cells (EPCs). Total mononuclear cells (MNCs) were isolated from peripheral blood by Ficoll density gradient centrifugation. EPCs were identified as adherent cells double positive for DiLDL-up-take and lectin binding by direct fluorescent demonstrated under a laser scannin...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد تهران مرکزی - دانشکده برق و الکترونیک 1390

there are many approaches for solving variety combinatorial optimization problems (np-compelete) that devided to exact solutions and approximate solutions. exact methods can only be used for very small size instances due to their expontional search space. for real-world problems, we have to employ approximate methods such as evolutionary algorithms (eas) that find a near-optimal solution in a r...

A.R. Nikoofar, B. Goliaei, Gh.R. Fazeli, S. Khoei,

Background: Several cell lines when cultured under proper condition can form three dimensional structures called multicellular tumor spheroids. Tumor spheroids are valuable in vitro models for studying physical and biological behavior of real tumors. A number of previous studies using a variety of techniques have shown no relationship between radiosensitivity and DNA strand breaks in monolayer ...

Journal: :Okayama Igakkai Zasshi (Journal of Okayama Medical Association) 1993

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