Collagen is the main structural protein of most hard and soft tissues in animals and the human body, which plays an important role in maintaining the biological and structural integrity of the extracellular matrix (ECM) and provides physical support to tissues. Collagen can be extracted and purified from a variety of sources and offers low immunogenicity, a porous structure, good permeability, ...

Collagen is frequently advocated as a scaffold for use in regenerative medicine. Increasing the mechanical stability of a collagen scaffold is widely achieved by cross-linking using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). However, this treatment consumes the carboxylate-containing amino acid sidechains that are crucial for recognition by the cell-surf...

The shape, cellular arrangement and the tensile strength of organs are determined by their extracellular matrix (ECM) [l]. This consists of a variety of molecules, among which fibrillar collagens and proteoglycans quantitatively predominate. The cells of the connective tissue were formerly considered to be almost metabolically inactive, but are now recognised as being an active participant in t...

Objective: To evaluate the regeneration of mandibular cartilage defect after implantation human umbilical cord mesenchymal stem cells (hUCMSC) over platelet rich fibrin (PRF) as scaffold. Material and Methods: 20 male Wistar rats were randomly divided into four experimental groups consisting of: a control group featuring untreated defects (C), whose implanted with hUCMSC (E1), PRF (E2), scaffol...

An important element of artificial skin is a tissue scaffold that allows for fast host regeneration. We present a microfabrication strategy, based on gelling collagen-based components inside a microfluidic device, that produces well-controlled pore sizes inside the scaffold. This strategy can produce finely patterned tissue scaffolds of clinically relevant dimensions suitable for surgical handl...

Collagen and hyaluronic acid (HA) are main components of the extracellular matrix and have been utilized in electrospinning; a technique that creates nanosized fibers for tissue scaffolds. A collagen/HA polymer solution was electrospun into a scaffold material for osteoporosis patients who have reduced bone strength. To synthesize nanofibers, a high voltage was applied to the polymer solution t...

please cite this article as : tavakoli-darestani r, kazemian gh, emami m, kamrani-rad a. poly (lactide-co-glycolide) nanofibers coated with collagen and nano-hydroxyapatite for bone tissue engineering. novel biomed 2013;1:8-15. background: a combination of polymeric nanofibrous scaffold and bioactive materials is potentially useful in bone regeneration applications. materials and methods: in th...

Changmin Hu, Mei Wei. Department of Materials Science and Engineering, Institute of Materials Science, University of Connecticut, Storrs, CT 06269, USA. Statement of Purpose: Natural bone is an outstanding organic-inorganic nanocomposites consisting primarily of collagen fibrils and hydroxyapatite (HA) crystals, which provides skeletal and metabolic functions of the natural bone. Many researche...

Coherent anti-Stokes Raman scattering (CARS) microscopy is combined with second harmonic generation (SHG) technique in order to follow the early stage of stem cell differentiation within a 3D scaffold. One of the first evidence of hMSCs differentiation is the formation of an extracellular matrix (ECM) where the collagen protein is its main component. This work demonstrated the multimodal CARS a...

Tissue-engineered skin with mechanical and biological properties that match the native tissue could be a valuable graft to treat non-healing chronic wounds. Fibroblasts grown on a suitable biodegradable scaffold are a feasible strategy for the development of a dermal substitute above which epithelialization may occur naturally. Cell growth and phenotype maintenance are crucial to ensure the fun...